Leishmania (Leishmania) amazonensis (MHOM/BR/1989/166MJO) was used in promastigote forms, in the stationary growth phase (day 5 of culture). The parasites were obtained from popliteal lymph nodes of L. amazonensis-infected BALB/c mice and maintained in 199 culture medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 10 mM HEPES Biological Buffer (AMRESCO), 0.1% human urine, 0.1% L-glutamine (SYNTH), penicillin (10 U/mL) and streptomycin (10 μg/mL) (Gibco), and 10% sodium bicarbonate (SYNTH). Cell cultures were incubated at 25°C in 25 cm2 flasks. All parasites were from a culture that was serially passed for less than 5 weeks.
Hepes biological buffer
Manufactured by Avantor
HEPES Biological Buffer is a chemical compound commonly used in biological research and laboratory applications. It serves as a pH buffering agent, maintaining a stable and optimal pH environment for various biological processes and experiments.
Automatically generated - may contain errors
Lab products found in correlation
199 culture medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
2 protocols using hepes biological buffer
1
Cultivation of Leishmania amazonensis Promastigotes
Check if the same lab product or an alternative is used in the 5 most similar protocols
Leishmania (Leishmania) amazonensis (MHOM/BR/1989/166MJO) was used in promastigote forms, in the stationary growth phase (day 5 of culture). The parasites were obtained from popliteal lymph nodes of L. amazonensis-infected BALB/c mice and maintained in 199 culture medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 10 mM HEPES Biological Buffer (AMRESCO), 0.1% human urine, 0.1% L-glutamine (SYNTH), penicillin (10 U/mL) and streptomycin (10 μg/mL) (Gibco), and 10% sodium bicarbonate (SYNTH). Cell cultures were incubated at 25°C in 25 cm2 flasks. All parasites were from a culture that was serially passed for less than 5 weeks.
+ Expand
Leishmania (Leishmania) amazonensis (MHOM/BR/1989/166MJO) was used in promastigote forms, in the stationary growth phase (day 5 of culture). The parasites were obtained from popliteal lymph nodes of L. amazonensis-infected BALB/c mice and maintained in 199 culture medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 10 mM HEPES Biological Buffer (AMRESCO), 0.1% human urine, 0.1% L-glutamine (SYNTH), penicillin (10 U/mL) and streptomycin (10 μg/mL) (Gibco), and 10% sodium bicarbonate (SYNTH). Cell cultures were incubated at 25°C in 25 cm2 flasks. All parasites were from a culture that was serially passed for less than 5 weeks.
2
Cultivation of L. amazonensis Promastigotes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Leishmania (Leishmania) amazonensis (MHOM/BR/1989/166MJO) was used in promastigote forms, in the stationary growth phase (day 5 of culture). The parasites were obtained from popliteal lymph nodes of L. amazonensis-infected BALB/c mice and maintained in 199 culture medium (GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO), 1M HEPES Biological Buffer (AMRESCO), 1% human urine, 1% L-glutamine (SYNTH), penicillin (10 U.mL-1) and streptomycin (10 μg.mL-1) (GIBCO) and 10% sodium bicarbonate (SYNTH). Cell cultures were incubated at 25°C in 25-cm2 flasks. All parasites were from a culture that was serially passed for less than 5 weeks.
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