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Antibodies against inos

Manufactured by Santa Cruz Biotechnology

Antibodies against iNOS (inducible Nitric Oxide Synthase) are laboratory reagents used to detect and analyze the expression of the iNOS enzyme. iNOS is an enzyme involved in the production of nitric oxide, which plays a role in various biological processes. These antibodies can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of iNOS in cells and tissues.

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2 protocols using antibodies against inos

1

Signaling Pathway Antibody Protocol

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Antibodies against JNK (cat. no. sc-7345), p-JNK (cat. no. sc-293136), ERK (cat. no. sc-514302), p-ERK (cat. no. sc-81492), NF-κB (cat. no. sc-8008), p-NF-κB (cat. no. sc-136548), p-IκB (cat. no. sc-8404), and β-actin (cat. no. sc-8432) were purchased from Santa Cruz Biotechnology, Inc. Antibodies against iNOS (cat. no. 13120) and COX-2 (cat. no. 4842) were acquired from Cell Signaling Technology, Inc. The Qunati-MAX™ WST-8 Cell Viability Assay Kit and WestGlow™ Chemiluminescent substrate were obtained from Biomax FBS, RIPA buffer and DMEM were purchased from Gibco; Thermo Fisher Scientific, Inc. Penicillin/streptomycin antibiotics, carboxy-H2DCFDA and Goat anti-Mouse IgG Alexa Fluor 488 (cat. no. A-11001) were purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Thermo Fisher Scientific, Inc.). Griess reagent and lipopolysaccharide (LPS, cat. no. L2630) were procured from Sigma-Aldrich; Merck KGaA. ELISA kits for IL-6 and TNF-α were obtained from R&D Systems, Inc. The Bradford assay reagent and SDS-PAGE sample loading buffer were purchased from Bio-Rad Laboratories, Inc.
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2

Zymosan-Induced Inflammatory Signaling

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Zymosan and agmatine were obtained from Sigma-Aldrich. Antibodies against iNOS, NF-κB p65, IκB-α, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA), and those against phosphor (p)-IκB and NF-κB p65 were from Cell Signaling Tech (Danvers, MA). Mouse TNF-α, IL-1β, and IL-6 enzyme-lined immunosorbent assay (ELISA) kits were purchased from Boster Biotechnology (Wuhan, China). All suspensions were freshly made before use.
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