Blood containing ∼50,000 parasites of the ΔSAS44 line was injected i.p. into mice to initiate infections. Asexual stages and gametocyte production were monitored by microscopy on Giemsa-stained thin smears. 4–5 d post-infection, exflagellation and ookinete conversion were examined as described previously (Zeeshan et al, 2019b (link)) with a Zeiss AxioImager M2 microscope (Carl Zeiss, Inc.) fitted with an AxioCam ICc1 digital camera. To analyse mosquito transmission, 30–50 Anopheles stephensi SD 500 mosquitoes were allowed to feed for 20 min on anaesthetized, infected mice with an asexual parasitaemia of 15% and a comparable number of gametocytes as determined on Giemsa-stained blood films. To assess midgut infection, ∼15 guts were dissected from mosquitoes on day 14 post-feeding, and oocysts were counted on an AxioCam ICc1 digital camera fitted to a Zeiss AxioImager M2 microscope using a 63× oil immersion objective. On day 21 post-feeding, another 20 mosquitoes were dissected, and their guts crushed in a loosely fitting homogenizer to release sporozoites, which were then quantified using a haemocytometer. Mosquito bite back experiments were performed 21 d post-feeding using naive mice, and blood smears were examined after 3–4 d.
Axiocam icc1 digital
Manufactured by Zeiss
The AxioCam ICc1 is a digital camera designed for microscopy applications. It features a high-resolution CMOS sensor and delivers detailed, high-quality images. The camera is suitable for a wide range of microscopy techniques and can be integrated into various imaging systems.
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Lab products found in correlation
4 protocols using axiocam icc1 digital
1
Characterizing Malaria Parasite Transmission Dynamics
2
Visualizing Ookinete and Zygote Surface Proteins
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Images were captured using a 63× oil immersion objective on a Zeiss AxioImager M2 microscope fitted with an AxioCam ICc1 digital camera and analysed with the AxioVision 4.8.2 software. Ookinetes and zygotes were stained with a Cy3‐conjugated mouse monoclonal antibody 13.1, which recognises the P28 protein on the parasite surface (Tewari, Dorin, Moon, Doerig, & Billker, 2005 ).
3
Rodent Malaria Transmission Assay
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Blood containing ~50,000 parasites of the PP1PTD line was injected intraperitoneally (i.p.) into mice to initiate infections. Asexual stages and gametocyte production were monitored by microscopy on Giemsa-stained thin smears. Four to five days post infection, exflagellation and ookinete conversion were examined with a Zeiss AxioImager M2 microscope (Carl Zeiss, Inc) fitted with an AxioCam ICc1 digital camera70 (link). To analyse mosquito transmission, 30–50 Anopheles stephensi SD 500 mosquitoes were allowed to feed for 20 min on anaesthetised, infected mice with an asexual parasitaemia of 15% and a comparable number of gametocytes as determined on Giemsa-stained blood films. To assess mid-gut infection, ~15 guts were dissected from mosquitoes on day 14 post feeding, and oocysts were counted on an AxioCam ICc1 digital camera fitted to a Zeiss AxioImager M2 microscope using a ×63 oil immersion objective. On day 21 post-feeding, another 20 mosquitoes were dissected, and their guts crushed in a loosely fitting homogenizer to release sporozoites, which were then quantified using a haemocytometer or used for imaging. Mosquito bite back experiments were performed 21-days post-feeding using naive mice, and blood smears were examined after 3–4 days.
4
Plasmodium Transmission Dynamics Evaluation
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Blood samples containing approximately 50,000 parasites of the ark2-knockdown/eb1 knockout lines were injected intraperitoneally (i.p.) into mice to initiate infection. Asexual stages and gametocyte production were monitored by microscopy on Giemsa-stained thin smears. Four to 5 days post infection, exflagellation and ookinete conversion were examined as described previously52 (link) with a Zeiss AxioImager M2 microscope (Carl Zeiss, Inc.) fitted with an AxioCam ICc1 digital camera. To analyse mosquito infection and transmission, 30 to 50 Anopheles stephensi SD 500 mosquitoes were allowed to feed for 20 min on anaesthetised, infected mice with at least 15% asexual parasitaemia and carrying comparable numbers of gametocytes as determined on Giemsa-stained blood films. To assess midgut infection, ~15 guts were dissected from mosquitoes on days 7 and 14 post feeding and oocysts were counted using a ×63 oil immersion objective. On day 21 post feeding, another 20 mosquitoes were dissected, and their guts and salivary glands crushed separately in a loosely fitting homogeniser to release sporozoites, which were then quantified using a haemocytometer or used for imaging. Mosquito bite-back experiments were performed 21 days post feeding using naive mice, and blood smears were examined after 3–4 days.
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