Cells were cultured in 24-well ultra-low attachment plates in a serum-free medium supplemented with 5 µg/ml insulin, 0.4% bovine serum albumin, 10 ng/ml basic fibroblast growth factor and 20 ng/ml human recombinant EGF for 6 days. The size and number of spheroids formed were analyzed using a light microscope (CKX31-11 PHP; Olympus). Sphere formation was determined by counting the number of spheroids with a size of >50 µm using NIS-Element F3.0 program (Nikon, Tokyo, Japan).
Nis element f3.0 program
Manufactured by Nikon
Sourced in Japan
NIS-Element F3.0 is a software program developed by Nikon for microscopy image acquisition, analysis, and processing. It provides a comprehensive suite of tools for researchers and scientists working with Nikon microscopes. The software enables users to capture high-quality images, perform measurements, and analyze various microscopic samples.
Automatically generated - may contain errors
Lab products found in correlation
Six well ultralow attachment plates, by Corning (2 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Ckx31 11 php, by Olympus (1 mentions)
Insulin, by Merck Group (1 mentions)
3 protocols using nis element f3.0 program
1
Serum-free Culture for Spheroid Formation
2
Spheroid Culture of Gastric Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transfected MKN‐45 and AGS cells were plated in the six‐well ultra‐low attachment plates (Corning). Cells (2 × 105) were cultured in the serum‐free DMEM medium containing 0.4% BSA (Sigma), 2% B27 (BD Pharmingen), 5 μg/mL insulin (Sigma), 20 ng/mL basic fibroblast growth factor (bFGF, Invitrogen) and 20 ng/mL epidermal growth factor (EGF, Invitrogen). Following incubation for 2 weeks at 37°C, the diameter and quantity of spheres were analysed with a light microscope (Nikon) and NIS‐Element F3.0 program (Nikon).
3
Sphere Formation Assay for Neural Progenitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (105) suspended in the serum-free DMEM medium containing 0.4% BSA (Sigma), 2% B27 (BD Pharmingen), 5 μg/mL insulin (Sigma), 20 ng/mL basic fibroblast growth factor (bFGF, Invitrogen) and 20 ng/mL epidermal growth factor (EGF, Invitrogen) were plated in the six-well ultra-low attachment plates (Corning). Two weeks later, images of the spheres were taken in three randomly chosen fields with the microscope (Nikon) and NIS-Element F3.0 program (Nikon) and the numbers of spheres in each field were counted for statistical analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!