Cells were washed with PBS, lysed in sample buffer, boiled for 3 min, resolved by SDS-PAGE and analyzed by immunoblotting using primary antibodies specific for human MX2 (sc-47197 (N-17), Santa Cruz Biotechnology), human TRIM5α (ab4389, Abcam), human PSMB8 (ab3329, Abcam), human PSMB9 (ab3328, Abcam), human PSMB10 (ab77735, Abcam), human PA28A (ab155091, Abcam), human PA28B (ab183727, Abcam), FLAG (HRP-conjugated M2, Sigma), HA (HRP-conjugated 3F10, Sigma) or human α-tubulin (DM1A, Sigma), and detected using either horseradish peroxidase-conjugated secondary antibodies and chemiluminescence (ECL+ western blotting substrate, Pierce) or IRDye®-800CW-labelled secondary antibodies and the LI-COR infrared imaging technology (LI-COR UK LTD).
Ab4389
Manufactured by Abcam
Ab4389 is a high-quality antibody produced by Abcam. It is designed for use in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab3328, by Abcam (2 mentions)
Ab77735, by Abcam (2 mentions)
Ab155091, by Abcam (2 mentions)
Ab183727, by Abcam (2 mentions)
Ab3329, by Abcam (2 mentions)
Sc 47197 n 17, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated 3f10, by Merck Group (2 mentions)
Infrared imaging technology, by LI COR (2 mentions)
2 protocols using ab4389
1
Immunoblotting Analysis of Immune Proteins
2
Immunoblotting Analysis of Immune Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were washed with PBS, lysed in sample buffer, boiled for 3 min, resolved by SDS-PAGE and analyzed by immunoblotting using primary antibodies specific for human MX2 (sc-47197 (N-17), Santa Cruz Biotechnology), human TRIM5α (ab4389, Abcam), human PSMB8 (ab3329, Abcam), human PSMB9 (ab3328, Abcam), human PSMB10 (ab77735, Abcam), human PA28A (ab155091, Abcam), human PA28B (ab183727, Abcam), FLAG (HRP-conjugated M2, Sigma), HA (HRP-conjugated 3F10, Sigma) or human α-tubulin (DM1A, Sigma), and detected using either horseradish peroxidase-conjugated secondary antibodies and chemiluminescence (ECL+ western blotting substrate, Pierce) or IRDye®-800CW-labelled secondary antibodies and the LI-COR infrared imaging technology (LI-COR UK LTD).
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