The degradation of the 3D-printed collagen-based gels with and without Ag was evaluated by collagenase digestion. Briefly, the Col and Col-Ag gels were first weighed and placed in a 24-well plate. Then, 500 μL of a 50 U/mL solution of type I collagenase enzyme (Gibco®, 340 U/mg) was added to each of the wells containing the 3D-printed scaffolds and incubated at 37 °C. The weight of the Col and Col-Ag gels was measured after 0.5 h, 1 h, 2 h, 3 h, 4 h, 5 h, and 24 h. For this, the material was removed from the enzyme solution, the excess of liquid was dried and finally weighted. The changes of weight of Col and Col-Ag gels were analyzed over time.
Type 1 collagenase enzyme
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Type I collagenase enzyme is a proteolytic enzyme used to break down collagen, the primary structural protein in connective tissues. It is commonly used in cell isolation and tissue dissociation applications.
Automatically generated - may contain errors
Lab products found in correlation
Hyaluronidase enzyme, by Merck Group (2 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dioleoylphosphatidylethanolamine dope, by Avanti Polar Lipids (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Gm csf, by BioLegend (1 mentions)
Recombinant mouse il 4, by BioLegend (1 mentions)
Phytohemagglutinin (pha), by Thermo Fisher Scientific (1 mentions)
Cholesterol, by Merck Group (1 mentions)
4 protocols using type 1 collagenase enzyme
1
Collagenase Degradation of 3D-Printed Collagen Gels
2
Adipose-Derived Stem Cell Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ADSCs were prepared from liposuctioned human adipose tissue. 60 g of adipose tissue sample was transferred to the culture room in a sterile container. In order to remove blood cells, fat samples were washed several times with Phosphate-Buffered Saline (PBS) solution (Shellmax) and penicillin and streptomycin (Gibco) antibiotics were added. The adipose tissue was then divided into small pieces and mixed with 3 ml of type I collagenase enzyme (0.075%) (Gibco) dissolved in PBS. After digestion, the small pieces were placed in a 37-degree incubator for 40 minutes, during which time the sample was shaken every 8 minutes for further digestion of the tissue.
The sample was then divided into two parts, one was added to DMEM (Gibco) medium enriched with 10% HS (human serum) and the other to DMEM (Gibco) medium enriched with 10% FBS (animal serum). The cells were then centrifuged at 1200 rpm for 6 minutes. After the addition of penicillin and streptomycin antibiotics to the solution containing adipose tissue mesenchymal cells. Finally, cultures containing HS and FBS were incubated at 37 ° C with 5% CO2 and 90% humidity.
It is noteworthy that adipose tissue was collected after receiving written consent from the volunteer of lipolysis and this operation was performed by a surgeon in the operating room under completely sterile conditions.
The sample was then divided into two parts, one was added to DMEM (Gibco) medium enriched with 10% HS (human serum) and the other to DMEM (Gibco) medium enriched with 10% FBS (animal serum). The cells were then centrifuged at 1200 rpm for 6 minutes. After the addition of penicillin and streptomycin antibiotics to the solution containing adipose tissue mesenchymal cells. Finally, cultures containing HS and FBS were incubated at 37 ° C with 5% CO2 and 90% humidity.
It is noteworthy that adipose tissue was collected after receiving written consent from the volunteer of lipolysis and this operation was performed by a surgeon in the operating room under completely sterile conditions.
3
Lipid-Based Nanoparticle Formulation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EPA was purchased from MetonChem (China). Hydrogenated soy phosphatidylcholine (HSPC), cholesterol (Chol), mPEG2000-distearoylphosphatidyl-ethanolamine (mPEG2000-DSPE), N-[1-(2, 3-Dioleoyloxy) propyl]-N, N, N-trimethylammoniummethyl-sulfate (DOTAP) and dioleoylphosphatidylethanolamine (DOPE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Hyaluronidase enzyme was purchased from Sigma-Aldrich (Steinheim, Germany). Collagenase Type I enzyme was purchased from Gibco (UK). Phytohemagglutinin (PHA), Calcein AM (AM = acetoxymethyl) were purchased from Invitrogen (Carlsbad, CA). All flow cytometry antibodies and kits were purchased from BD Biosciences (SanDiego, USA). All solvents and materials were purchased from Merck (Germany) and were in the analytical grade.
4
Lipid-based Nanoparticle Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1, 2-distearoyl-Sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] (DSPEPEG2000-Maleimide) and N-[1-(2, 3-Dioleoyloxy) propyl]-N, N, N-trimethylammoniummethyl-sulfate (DOTAP) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). Cholesterol, Hyaluronidase enzyme, and Lipopolysaccharides (LPS) were purchased from Sigma-Aldrich (Steinheim, Germany). Recombinant Mouse IL-4 and GM-CSF were purchased from Biolegend (San Diego, USA). Calcein AM (AM = acetoxymethyl) and Phytohemagglutinin (PHA) were purchased from Invitrogen (Carlsbad, CA). Collagenase Type I enzyme was purchased from Gibco (UK). Flow cytometry kits and antibodies were purchased from BD Biosciences (San Diego, USA). All other solvents and reagents were used as a chemical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!