A1 hd

Detection of JAK1 and JAK3 in MSG. FFPE MSG sections were processed following standard procedures. Sections were blocked, incubated in antibodies, and mounted (supplemental table 2). Immunofluorescence in primary cell culture assays. Primary salivary gland epithelial cells (pSGECs) were plated on chamber slides and stimulated as indicated, then stained with antibodies (supplemental table 2). Images were acquired on Nikon A1 HD (Nikon) confocal microscope and processed with CellProfiler in ImageJ (Broad Institute).^{11 (link)}

THP1 Macrophages and NS-SV-TTAC Acinar Cells were plated on an 8-well Lab-Tek II Chamber Slide (Thermo Scientific, 154534). After indicated treatment, cells were loaded with Quant-iT PicoGreen Reagent (1:50) (Invitrogen, #P7589A) and MitoTracker Red CMXRos (Invitrogen, #M7512) (1:10000) and incubated for 45 minutes at 37°C. The cells were fixed for 20 minutes at room temperature in 4% PFA. The cells were blocked in 3% BSA diluted in 1X PBS for 1 hour. Cells were washed with 1X PBS and then stained with primary antibodies overnight and then stained by secondary antibodies for 1 hour at room temperature (Supplemental Table 2). Images were acquired on Nikon A1 HD (Nikon) confocal microscope and processed with CellProfiler in ImageJ (Broad Institute).