The ALP biochemical parameter was determined in sera of mice immunized with T4, AAV, or T4-AAV. Naïve mice were used as control. ALP level was measured using a commercial ALP assay kit (Elabscience, MD) following the manufacturer’s instructions.
Alp assay kit
Manufactured by Elabscience
Sourced in United States
The ALP assay kit is a laboratory product designed to measure the activity of the enzyme alkaline phosphatase (ALP) in biological samples. It provides a quantitative assessment of ALP levels, which can be useful in various clinical and research applications.
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3 protocols using alp assay kit
1
Measuring Alkaline Phosphatase in Immunized Mice
2
Asparagusic Acid Impacts Protoscolex ALP
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The ALP levels in the culture supernatant of the protoscoleces after asparagusic acid intervention were determined using an ALP assay kit (Elabscience, Wuhan, China) according to the manufacturer’s instructions. After the protoscoleces underwent the intervention with different concentrations of asparagusic acid for 48 h, the culture supernatant was collected and centrifuged at 10,000× g for 10 min at 4 °C, and the supernatant was taken for ALP determination.
3
Quantifying Cellular ALP Activity
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ALP activity was determined using an ALP assay kit (ElabScience, USA). After cells were captured, they were lysed with a lysis buffer. The supernatant was recovered after centrifugation and transferred to a 96-well plate. The supernatant was then incubated with p-nitrophenyl phosphate (PNPP) and reaction buffer for 10 min at 37 °C. Finally, the absorbance value was determined at 570 nm by enzyme labeling.
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