Sodium selenite (Na2SeO3) and ascorbic acid (vitamin C) were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, PR China). Hoechst 33342 and the Annexin V-FITC Apoptosis Kit were purchased from Sinopharm (Shanghai, PR China). The TUNEL assay kit and cy5.5 were purchased from Beyotime (Shanghai, PR China). The cyclic polypeptide Arg-Gly-Asp-D-Phe-Cys (RGDfC) was obtained from China Polypeptides Co., Ltd. All the antibodies were purchased from CST (Massachusetts, US). The siRNA was obtained from RiboBio (Guangzhou, PR China), and the HES5-siRNA sequence was as follows: 5′-AAGGCTACTCGTGGTGCCT-3'.
Hoechst 33342
Manufactured by Sinopharm
Sourced in China
Hoechst 33342 is a fluorescent dye that binds to DNA. It has an excitation wavelength of 355 nm and an emission wavelength of 461 nm. Hoechst 33342 is commonly used for cell staining and nucleic acid detection applications.
Automatically generated - may contain errors
Lab products found in correlation
Tunel assay kit, by Beyotime (1 mentions)
Ascorbic acid, by Guangzhou Chemical (1 mentions)
Sodium selenite na2seo3, by Guangzhou Chemical (1 mentions)
Annexin 5 fitc apoptosis kit, by Sinopharm (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Beyotime (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
O phenylenediamine, by Merck Group (1 mentions)
α tocopheryl succinate, by Merck Group (1 mentions)
Mitochondrial membrane potential assay kit with jc 1, by Beyotime (1 mentions)
Fluorescein isothiocyanate (fitc), by Sinopharm (1 mentions)
2 protocols using hoechst 33342
1
Apoptosis Induction via Selenium and Vitamin C
2
Evaluating CDDP-Induced Cytotoxicity in A549 Cells
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CDDP was purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). α-tocopheryl succinate (TOS), AgNO3 and O-phenylenediamine (OPDA) were obtained from Sigma-Aldrich Co., Ltd. (Steinheim, Germany). Fluorescein isothiocyanate (FITC) was purchased from Sinopharm Chemical Reagent Co., Ltd. Hoechst 33342, 2′,7′-dichlorofluorescein diacetate (DCFH-DA), Annexin V-FITC/PI Apoptosis Detection Kit and Mitochondrial membrane potential assay kit with JC-1 were all purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum and penicillin-streptomycin were ordered from Life Technologies Corporation (Gibco, USA) and directly used for cells tests. Human non-small cell lung cancer cell line (A549) was purchased from the Chinese Academy of Science cells Bank (Shanghai, China).
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