Isoflavone

Manufactured by Merck Group

Sourced in United States

Isoflavones are a class of organic compounds found in various plants, particularly soybeans. They are commonly used in laboratory applications for analysis and research purposes. Isoflavones possess unique chemical properties that make them valuable tools for scientists and researchers working in fields such as agriculture, food science, and pharmaceutical development.

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Isoflavone kaempferol (4 citations) Isoflavone standards (3 citations)

4 protocols using isoflavone

Evaluating Isoflavone's Neuroprotective Effects

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Both naive and differentiated PC12 cells were cultured at 37˚C in RPMI 1640 media (Sigma USA) supplemented with 10% FBS, 2 mM L-glutamine, 100 IU/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere containing 5% carbon dioxide in room air. The dispersed cells were seeded onto colla-gen-coated 96-well plates at a density of 3 × 10 4 cells/well, and cultured under various drug regimen combinations. The cultured cells were divided into five groups including control (vehicle), MPP + (250 mmol/L), Isoflavone (Guangzhou Aipu technology company) combined with MPP + (10 mM Isoflavone and 250 mmol/L MPP + ), and Isoflavone alone groups (10 mM), SN-50 + MPP + (Sigma USA) group (10 mM SN-50 and 250 mmol/L MPP + ).

Cheng W., Huang A., Zhang L., Feng D., Sun X., Xu H., Luo D., & Li X. (2020). Isoflavone Attenuates the Nuclear Transcription Factor Kappa B (NF-<i>κ</i>B) Activation on MPP<sup>+</sup>-Induced Apoptosis of PC12 Cells. Journal of Behavioral and Brain Science, 10(05), 191-199.

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Lipid Bilayer Characterization with Phytochemicals

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CaCl₂, NaCl, NaOH, calcein, Triton X-100, Sephadex G-50, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), dimethylsulfoxide (DMSO), and plant polyphenols: chalcones (4′-hydroxychalcone (≥99%, high-performance liquid chromatography (HPLC)), cardamonin (≥98%, HPLC), iso liquiritigenin (≥98%, HPLC)), dihydrochalcones (phloretin (≥99%, HPLC)), stilbenes (resveratrol (≥99%, HPLC), piceatannol (≥98%, HPLC)), isoflavones (daidzein (≥98%, HPLC), biochanin A (≥95%, HPLC), genistein (≥98%, HPLC), genistin (≥95%, HPLC)), flavanones (liquiritigenin (≥97%, HPLC), naringenin (≥95%, HPLC)), flavan-3-ols (catechin (≥98%, HPLC)), flavononols (taxifolin (≥90%, HPLC)), flavonols (quercetin, myricetin, and rutin), and lignans (honokiol (≥98%, HPLC)) were purchased from Sigma-Aldrich Company Ltd. (Gillingham, United Kingdom).
Lipids synthetic 1,2-dioleoyl-sn-glycero-3-phospho-(1-rac-glycerol) (DOPG), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phospho-(1-rac-glycerol) (DPPG), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and cholesterol (CHOL) were obtained from Avanti Polar Lipids (Avanti Polar Lipids, Inc., Alabaster, AL, USA).

Zlodeeva P.D., Shekunov E.V., Ostroumova O.S, & Efimova S.S. (2023). The Degree of Hydroxylation of Phenolic Rings Determines the Ability of Flavonoids and Stilbenes to Inhibit Calcium-Mediated Membrane Fusion. Nutrients, 15(5), 1121.

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Isoflavones Extraction and Identification

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Isoflavones, genistein and daidzein, were purchased from Sigma-Aldrich (St Louis, MO, USA). Dalbergioidin, 2′-hydroxygenistein, coumestrol, phaseollidin, phaseollinisoflavan, kievitone and phaseollin were obtained and identified from a previous work described by Durango et al. [22 ]. Methyl jasmonate (MeJA), palladium on carbon and oxalyl chloride was acquired from Sigma-Aldrich Co. (St Louis, MO, USA), whereas jasmonic acid (JA), anhydrous aluminum chloride (AlCl₃) and 2-carboxycinnamic acid were bought in Alfa Aesar Co. (Ward Hill, MA, USA). 1-oxo-4-indanoyl carboxylic acid was prepared by conventional organic reactions [23 (link)].

Botero L., Vizcaíno S., Quiñones W., Echeverri F., Gil J, & Durango D. (2021). Increased accumulation of isoflavonoids in common bean (Phaseolus vulgaris L.) tissues treated with 1-oxo-indane-4-carboxylic acid derivatives. Biotechnology Reports, 29, e00601.

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Antioxidant Activity of Soybean Extracts

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Soybean seeds (Dongsheng No. 1), harvested in 2019 in Heilongjiang province, China, were stored at −20 °C before use. Methanol (AR), ethyl acetate, 2,2’-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), nicotinamide adenine dinucleotide phosphate (NADPH), cinnamic acid were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Mercaptoethanol, coenzyme A (CoA), trolox, acetonitrile, methanol (HPLC grade) were purchased from Maclean Biotechnology Co., Ltd. (Shanghai, China). The standards of phenolic acids and isoflavone were purchased from Sigma-Aldrich Chemical Co. (Shanghai, China).

Zhao Y., Xie C., Wang P., Gu Z, & Yang R. (2021). GABA Regulates Phenolics Accumulation in Soybean Sprouts under NaCl Stress. Antioxidants, 10(6), 990.

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