Peripheral blood mononuclear cells were isolated from whole blood by Lymphoprep (Axis Shield, Dundee, Scotland), before positive selection of CD8+ T cells (EasySep™ Human CD8+ Selection Kit, STEMCELL Technologies, Vancouver, Canada) followed by negative selection of CD4+ T cells (EasySep™ Human CD4+ T cell Isolation kit, STEMCELL Technologies). Cells that achieved cell purity of more than 95% as measured by flow cytometry (Attune Acoustic Focusing Flow Cytometer, Life Technologies, Carlsbad, CA, USA) were included in the study. Two CD8+ T cell samples from MS patients did not reach 95% cell purity and were excluded from the analyses. Antibodies used for flow cytometry analyses were fluorescein isothiocyanate-conjugated mouse anti-human CD4 (clone RTF-4 g, Southern Biotech, Birmingham, AL, USA), mouse anti-human CD8 (clone HIT8a, BD biosciences, San Jose, CA, USA) and mouse IgG1 isotype control (15H6, Southern Biotech).
Mouse anti human cd8 clone hit8a
Manufactured by BD
Sourced in United States
The mouse anti-human CD8 (clone HIT8a) is a primary antibody that binds to the CD8 antigen expressed on the surface of human T cells. It is used for the identification and enumeration of CD8+ T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Attune acoustic focusing flow cytometer, by Thermo Fisher Scientific (2 mentions)
Mouse igg1 isotype control 15h6, by Southern Biotech (2 mentions)
Easysep human cd4 t cell isolation kit, by STEMCELL (1 mentions)
Lymphoprep, by Axis-Shield (1 mentions)
Easysep human cd8 selection kit, by STEMCELL (1 mentions)
Easysep human cd8 positive selection kit, by STEMCELL (1 mentions)
Fluorescein isothiocyanate conjugated mouse anti human cd4 clone rtf 4 g, by Southern Biotech (1 mentions)
2 protocols using mouse anti human cd8 clone hit8a
1
Isolation and Purification of CD8+ T Cells
2
Isolation and Characterization of CD4+ and CD8+ T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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+ and CD8 + T cells CD4 + and CD8 + T cells were purified using immunomagnetic cell separation kits (Human CD4 + negative selection kit and EasySep™ Human CD8 + positive selection kit and EasySep™, STEMCELL Technologies, Vancouver, Canada) as described in (Rhead et al., 2018; Bos et al., 2015) . Purity of isolated T cells was confirmed to be at least 95% as measured by flow cytometry (Attune Acoustic Focusing Flow Cytometer, Life Technologies, Carlsbad, CA, USA). Antibodies used for purity assessment by flow cytometry were fluorescein isothiocyanateconjugated mouse anti-human CD4 (clone RTF-4 g, Southern Biotech, Birmingham, AL, USA), mouse anti-human CD8 (clone HIT8a, BD Biosciences, San Jose, CA, USA) and mouse IgG1 isotype control (15H6, Southern Biotech).
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