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Photometrics evolve 512 emccd

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The Photometrics Evolve 512 EMCCD is a high-performance electron-multiplying charge-coupled device (EMCCD) camera. It is designed for applications that require high sensitivity, low-light detection, and fast image acquisition. The camera features a 512 x 512 pixel sensor with a pixel size of 16 x 16 micrometers, and it supports a range of readout speeds and electron-multiplying gain settings to optimize performance for different experimental requirements.

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Lab products found in correlation

Metamorph 7, by Molecular Devices (3 mentions) Eclipse ti e, by Nikon (3 mentions) Cfi apo tirf 100 1.49 n a oil objective, by Nikon (2 mentions) Inubg2e zilcs, by Tokai Hit (2 mentions) Evolve 512 camera, by Nikon (2 mentions) Optosplit 3 beamsplitter, by Cairn Research (2 mentions) Cfi apo tirf 100x 1.49 n a oil objective, by Nikon (1 mentions)

Close spelling variants of this product

Evolve 512 (7 citations) Photometrics Evolve 512 EMCCD camera (2 citations)

3 protocols using photometrics evolve 512 emccd

1

Microtubule Dynamics in EGFR Mutants

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MaMTH reporter HEK293 cells stably expressing EGFR WT or EGFR L858R/T790M/C797S were transfected with EB3-TagRFP. Cell were stimulated with 0.5 µg/ml tetracycline to induce EGFR expression 18 hours prior to imaging. Cells were incubated with EMI for 30 min prior to imaging. Inverted research microscope Nikon Eclipse Ti-E (Nikon) supplemented with the perfect focus system (PFS) (Nikon) and equipped with Nikon CFI Apo TIRF 100x 1.49 N.A. oil objective (Nikon), Photometrics Evolve 512 EMCCD (Roper Scientific) controlled with MetaMorph 7.7 software (Molecular Devices) was used to perform the live cell imaging. Images were acquired in a stream mode with an exposure time of 500 ms. Kymographs were generated using ImageJ plugin KymoResliceWide (https://github.com/ekatrukha/KymoResliceWide). Parameters of microtubule dynamics were analyzed as described previously41 ,42 .
Saraon P., Snider J., Kalaidzidis Y., Wybenga-Groot L.E., Weiss K., Rai A., Radulovich N., Drecun L., Vučković N., Vučetić A., Wong V., Thériault B., Pham N.A., Park J.H., Datti A., Wang J., Pathmanathan S., Aboualizadeh F., Lyakisheva A., Yao Z., Wang Y., Joseph B., Aman A., Moran M.F., Prakesch M., Poda G., Marcellus R., Uehling D., Samaržija M., Jakopović M., Tsao M.S., Shepherd F.A., Sacher A., Leighl N., Akhmanova A., Al-awar R., Zerial M, & Stagljar I. (2020). A drug discovery platform to identify compounds that inhibit EGFR triple mutants. Nature chemical biology, 16(5), 577-586.
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2

TIRF Microscopy for Live-Cell Imaging

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TIRFM was performed on an inverted research microscope Nikon Eclipse Ti-E (Nikon) with the perfect focus system (PFS) (Nikon), equipped with the Nikon CFI Apo TIRF 100× 1.49 N.A. oil objective (Nikon), Photometrics Evolve 512 EMCCD (Roper Scientific) and controlled with the MetaMorph 7.7 software (Molecular Devices). Images were projected onto the chip of Evolve 512 camera with intermediate lens 2.5X (Nikon C mount adapter 2.5X). To keep in vitro samples at 30°C, we used stage top incubator INUBG2E-ZILCS (Tokai Hit).
For excitation we used 491nm 100mW Stradus (Vortran), 561nm 100mW Jive (Cobolt) and 642 nm 110 mW Stradus (Vortran). We used ET-GFP 49002 filter set (Chroma) for imaging of proteins tagged with GFP, ET-mCherry 49008 filter set (Chroma) for imaging X-Rhodamine labelled tubulin or mCherry-EB3 and ET-405/488/561/647 for imaging SNAP-Alexa647. For simultaneous imaging of green and red fluorescence, we used the triple-band TIRF polychroic ZT405/488/561rpc (Chroma) and the triple-band laser emission filter ZET405/488/561m (Chroma), mounted in the metal cube (Chroma, 91032) together with Optosplit III beamsplitter (Cairn Research Ltd, UK) equipped with a double emission filter cube configured with ET525/50m, ET630/75m and T585LPXR (Chroma). We used sequential acquisition for triple colour imaging experiments.
Atherton J., Jiang K., Stangier M.M., Luo Y., Hua S., Houben K., van Hooff J.J., Joseph A.P., Scarabelli G., Grant B.J., Roberts A.J., Topf M., Steinmetz M.O., Baldus M., Moores C.A, & Akhmanova A. (2017). A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. Nature structural & molecular biology, 24(11), 931-943.
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3

TIRF Microscopy for Live-Cell Imaging

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TIRFM was performed on an inverted research microscope Nikon Eclipse Ti-E (Nikon) with the perfect focus system (PFS) (Nikon), equipped with the Nikon CFI Apo TIRF 100× 1.49 N.A. oil objective (Nikon), Photometrics Evolve 512 EMCCD (Roper Scientific) and controlled with the MetaMorph 7.7 software (Molecular Devices). Images were projected onto the chip of Evolve 512 camera with intermediate lens 2.5X (Nikon C mount adapter 2.5X). To keep in vitro samples at 30°C, we used stage top incubator INUBG2E-ZILCS (Tokai Hit).
For excitation we used 491nm 100mW Stradus (Vortran), 561nm 100mW Jive (Cobolt) and 642 nm 110 mW Stradus (Vortran). We used ET-GFP 49002 filter set (Chroma) for imaging of proteins tagged with GFP, ET-mCherry 49008 filter set (Chroma) for imaging X-Rhodamine labelled tubulin or mCherry-EB3 and ET-405/488/561/647 for imaging SNAP-Alexa647. For simultaneous imaging of green and red fluorescence, we used the triple-band TIRF polychroic ZT405/488/561rpc (Chroma) and the triple-band laser emission filter ZET405/488/561m (Chroma), mounted in the metal cube (Chroma, 91032) together with Optosplit III beamsplitter (Cairn Research Ltd, UK) equipped with a double emission filter cube configured with ET525/50m, ET630/75m and T585LPXR (Chroma). We used sequential acquisition for triple colour imaging experiments.
Atherton J., Jiang K., Stangier M.M., Luo Y., Hua S., Houben K., van Hooff J.J., Joseph A.P., Scarabelli G., Grant B.J., Roberts A.J., Topf M., Steinmetz M.O., Baldus M., Moores C.A, & Akhmanova A. (2017). A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. Nature structural & molecular biology, 24(11), 931-943.
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