N n diethyl p phenylenediamine

Manufactured by Merck Group

N,N-diethyl-p-phenylenediamine is a chemical compound used as a reagent in various analytical and testing applications. It is a colorless to light yellow crystalline solid with a distinct odor. The compound is soluble in water and organic solvents. N,N-diethyl-p-phenylenediamine is commonly used as an indicator in chemical analysis, specifically in the determination of oxidizing agents such as chlorine, chloramine, and hydrogen peroxide.

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P-phenylenediamine (70 citations) N,N-Diethyl-p-phenylenediamine sulfate (3 citations)

4 protocols using n n diethyl p phenylenediamine

Inactivation of Microorganisms by Free Chlorine

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Inactivation experiments by FC were conducted analogously to ClO₂ experiments. The initial FC concentrations ranged from 1 to 2 mg/L, and were prepared by diluting NaClO (13–14%, Reactorlab SA) in PBS (pH 7.4). Samples were taken periodically at time intervals of 10 or 15 s over the course of up to 90 s. The FC concentration (C_FC) was measured in every sample using the N,N-diethyl-p-phenylenediamine (Sigma-Aldrich) colorimetric method (Rice et al., 2012 ). The chlorine exposure was determined from the cumulative area under the curve of C_FC vs. t:
The inactivation rate constant k_FC was then determined using a first-order Chick-Watson model:

Zhong Q., Carratalà A., Ossola R., Bachmann V, & Kohn T. (2017). Cross-Resistance of UV- or Chlorine Dioxide-Resistant Echovirus 11 to Other Disinfectants. Frontiers in Microbiology, 8, 1928.

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Synthesis and Characterization of Theranostic Nanomaterials

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Iron(III) chloride hexahydrate (FeCl₃·6H₂O), chloroauric acid trihydrate, N,N′-diethyl-p-phenylenediamine
(DPD), BSA, and ICG (Cardiogeen) were purchased from Sigma-Aldrich.
Ethylene glycol (EG) and sodium acetate (NaAc) were obtained from
TCI Chemicals. Gelatine powder 240 Bloom (Bacto) was procured from
S.D. Fine-chem limited. Double distilled water was used for all synthetic
experiments. All the chemicals were of analytical grade and used as
received. MTT, bisbenzimide (Hoechst 33342), and phalloidin-TRITC
conjugate were purchased from Sigma-Aldrich. PLC/PRF/5 cell lines
were purchased from the National Center of Cell Science (NCCS, Pune,
India). Human umbilical vein endothelial cells (HUVECs) was purchased
from Lonza (Cat. no. 2517A) and maintained in endothelial growth medium
from Lonza (Cat. no. 3162). Dulbecco’s modified Eagle medium
(DMEM, HiMedia) was used in supplementation with 10% fetal bovine
serum (FBS, HiMedia) and 1% antibiotic/antimycotic solution (HiMedia).
Proteinase K was purchased from Ambion RNA by Life Technologies.

Yadav P., Chaturvedi S., Biswas S.K., Srivastava R., Kailasam K., Mishra A.K, & Shanavas A. (2022). Biodegradable Protein-Stabilized Inorganic Nanoassemblies for Photothermal Radiotherapy of Hepatoma Cells. ACS Omega, 7(10), 8928-8937.

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Oxidative Stress Biomarkers Quantification

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Glacial acetic acid (ID: CD3C630227) was obtained from Merck Specialties Pvt. Ltd., Mumbai, Ferrous sulphate (ID: 064963) from Sisco Research Laboratories Pvt. Ltd, Mumbai; N, N-diethyl-p-phenylenediamine (ID: 24855816) and Sodium Acetate (ID: 24899538) from Sigma-Aldrich, Mumbai, while ROS Elisa kit was procured from Elabscience (Lot No: AK0016FEB18047) and Human MDA Elisa kit (coated plate) was procured from Bioassay Technology Laboratory (Lot No: 20160203).

Gupta M., & Mondal A. (2020). CLINICAL ASSESSMENT OF HERBAL FORMULATION IN REDUCING OXIDATIVE STRESS CAUSING SPERMATOGENETIC DISORDERS USING THE ROS, D- ROMS AND MDA TESTS. International Journal of Medical and Biomedical Studies, 4(7).

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Covalent Functionalization of (6,5)-SWCNTs

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N,N-diethlyanimoaryl OCC were covalently functionalized to the purified (6,5)-SWCNTs via diazonium chemistry. N,N-diethyl-4-animobenzene tetrafluoroborate was freshly synthesized from N,N-diethyl-p-phenylenediamine (97%, Sigma-Aldrich) and nitrous acid following a modified literature method.⁵⁸ The purified SWCNT solution was diluted with 1% sodium dodecyl sulfate (≥99.0%, Sigma-Aldrich) and mixed with the synthesized diazonium salts at the salt to carbon of (6,5)-SWCNT molar ratio of 3.17 to 1. The SWCNT-diazonium mixture was illuminated with a mercury arc lamp (X-Cite 120Q, Excelitas) at room temperature. After 20 minutes of illumination, the diazonium reaction was quenched by diluting the SWCNT solution with 1.04% DOC solution. The functionalized SWCNT solution was ultrafiltrated using Amicon^® Ultra filters (100kDa MWKO).

Pasqualucci L., Migliazza A., Fracchiolla N., William C., Neri A., Baldini L., Chaganti R.S., Klein U., Küppers R., Rajewsky K, & Dalla-Favera R. (1998). BCL-6 mutations in normal germinal center B cells: Evidence of somatic hypermutation acting outside Ig loci. Proceedings of the National Academy of Sciences of the United States of America, 95(20), 11816-11821.

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