Dmem high glucose 4.5 g l medium

Normal human epidermal keratinocytes (NHEKs) obtained from PromoCell (Heidelberg, Germany) were cultured in Keratinocyte Growth Medium 2 (PromoCell). HaCaT, HaCaT-AHR-KO, HaCaT-CYP1A1-KO and HaCaT-NRF2-KO keratinocytes were cultured in DMEM low glucose (1 g/l) medium (PAN Biotech, Aidenbach, Germany) and supplemented with 10% FBS and antibiotics/antimycotics (PAN Biotech). Stable AHR-knockdown HaCaT keratinocytes (HaCaT-shAHR) and respective empty vector control cells (HaCaT-EV) were cultured in regular HaCaT medium supplemented with 0.68 mg/ml G418 (Carl Roth, Karlsruhe, Germany). MCF-7 and MCF-7-AHR-KO cells were cultured in DMEM high glucose (4.5 g/l) medium (PAN Biotech) supplemented with 10% FBS and antibiotics/antimycotics. The generation and characterization of HaCaT-shAHR and HaCaT-EV cells (Fritsche et al., 2007 (link)) and MCF-7-AHR-KO cells (Vogel et al., 2021 (link)) has been previously described. HepG2 cells were cultured in RPMI 1640 containing 10% FBS and antibiotics/antimycotics (PAN Biotech). All cells were kept in a humidified atmosphere of 5% CO₂ at 37 °C.