Fluorescence confocal microscopy for Figure 2—figure supplement 1A and Figure 6—figure supplements 1 , 2 was performed using a Zeiss Axio Imager with a Yokogawa spinning-disc confocal scanner. Fluorescence confocal microscopy for all other figures was performed using a custom-built inverted Zeiss Axio Observer with CSU-W1 Sora spinning disk scan head (Yokogawa), 1×/2.8× relay lens (Yokogawa), fast piezo z-drive (Applied Scientific Instrumentation), and a iXon Life 888 EMCCD camera (Andor). Samples were illuminated with 405/488/561/637 nm solid-state laser (Coherent), using a 405/488/561/640 transmitting dichroic (Semrock) and 624-40/692-40/525-30/445-45 nm bandpass filter (Semrock), respectively. Images from either microscope were taken with using Slidebook v6.0 software (Intelligent Imaging Innovations) using a 40×–1.3 NA/63×–1.4 NA objective (Zeiss) depending on sample.
624 40 692 40 525 30 445 45 nm bandpass filter
Manufactured by IDEX Corporation
The 624-40/692-40/525-30/445-45 nm bandpass filter is a type of optical filter that selectively transmits light within a specific wavelength range while blocking light outside of that range. The filter has a center wavelength of 624 nm, 692 nm, 525 nm, or 445 nm, and a bandwidth of 40 nm, 40 nm, 30 nm, or 45 nm, respectively. This filter is designed to isolate and transmit a specific color or wavelength of light, which may be useful in various scientific, industrial, or medical applications that require the separation and analysis of light at specific wavelengths.
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Lab products found in correlation
Csu w1 sora spinning disk scan head, by Yokogawa (2 mentions)
Ixon life 888 emccd camera, by Oxford Instruments (2 mentions)
561 640 transmitting dichroic, by IDEX Corporation (2 mentions)
Axio observer, by Zeiss (1 mentions)
Axio imager, by Yokogawa (1 mentions)
Spinning disc confocal scanner, by Yokogawa (1 mentions)
Zen imaging software, by Zeiss (1 mentions)
Lsm 880 airyscan, by Zeiss (1 mentions)
Axio observer, by Yokogawa (1 mentions)
2 protocols using 624 40 692 40 525 30 445 45 nm bandpass filter
1
Fluorescence Confocal Microscopy Protocol
2
Fluorescence Confocal Microscopy Protocols
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Fluorescence confocal microscopy was performed using two microscopes: 1) an inverted Zeiss Axio Observer with CSU-W1 Sora spinning disk scan head (Yokogawa), 1X/2.8x relay lens (Yokogawa), fast piezo z-drive (Applied Scientific Instrumentation), a iXon Life 888 EMCCD camera (Andor), and a 405/488/561/637nm solid-state laser (Coherent) with a 405/488/561/640 transmitting dichroic (Semrock) and 624-40/692-40/525-30/445-45nm bandpass filter (Semrock) respectively. Slidebook v6.0 software (Intelligent Imaging Innovations) was used for image capture, and a 63X-1.4NA objective (Zeiss) was used; 2) an inverted ZEISS LSM 880-AiryScan (Carl Zeiss) equipped with a 63X objective. ZEN imaging software (Carl Zeiss) was used for image capture, and images were subsequently processed by the ZEN Airyscan Processing method.
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