Mmp3 antibody

Cells were washed twice with ice-cold PBS and then lysed in RIPA buffer (Beyotime Biotechnology) mixed with PMSF (100×), protease inhibitor cocktail (100×, Cell Signaling Technology), and PhosSTOPEASYpack (Roche). The reaction mixture was centrifuged at 12,000 × g for 15 min to remove cell fragments. Primary antibodies used were GBP5 Polyclonal Antibody (Proteintech, 13220-1), MMP3 Antibody (Proteintech, 17873-1), Phospho-Src Antibody (Cell Signaling Technology, 2105S), Src Antibody (Cell Signaling Technology, 2108S), Phospho-p44/42 MAPK (Erk1/2) Antibody (Cell Signaling Technology, 4370S), p44/42 MAPK (Erk1/2) Antibody (Cell Signaling Technology, 4695S), and GAPDH Antibody (Proteintech, 60004-1).

CCL9, CCL15, CCR3, MMP14 and CD3 antibodies were purchased from Abcam (Cambridge, UK). Both F4/80 and Ly6B.2 antibodies were from Bio-Rad (Hercules, CA, USA). CCR1 antibody was purchased from Novus Biologicals (Littleton, CO, USA). MMP2 and MMP3 antibodies were obtained from Thermo Fisher Scientific (Walthem, MA, USA). In addition, MMP3 antibody was also purchased from ProteinTech (San Diego, CA, USA). Recombinant CCL9 was from PeproTech (Cranbury, NJ, USA). CCL9 neutralizing antibody and its isotype control antibody were purchase from R&D Systems (Minneapolis, MN, USA) and BioLegend (San Diego, CA, USA), respectively. N-Acetyl Cysteine was purchased from Sigma-Aldrich (St. Louis, MO, USA). Other reagents used are described in the specific experiment sections.