Ag rnaex pro reagent ag21101

The rice cultivar LinDao10 was used in this study and the seedlings were grown inside a greenhouse at 28 •C and 70%-80% humidity with a photoperiod of 16h light and 8 dark. The rice seedlings at 3-leaf-tage were inoculated with RBSDV and RSV by using viruliferous small brown planthoppers (SBPH, Laodelphax striatellus). The viruliferous SBPHs were feed using assayed seedlings for two days and were then removed. We used rice seedlings inoculated with non-viruliferous SBPHs as controls (CK) in this work.
The leaves with disease phenotype in experiment groups and health leaves in control were harvested at 30 days after the inoculation. Subsequently, all collected leaves were quickly frozen in liquid nitrogen, and then stored at -80 •C separately until use. We used the mixed leaf tissues collected from 10 plants of each group as a biological replicate, and each treatment had three biological replicates. AG RNAex Pro Reagent AG21101 (Accurate Biotechnology (Hunan) Co., Ltd) was used to extract the total RNA of individual samples, and the speci c primers for RBSDV and RSV were designed to con rm successfully virus infection in each virus-inoculated sample by using RT-PCR (Table S1).