Chemidocxrs gel imaging and analysis system

Donepezil hydrochloride table (Lot: 110822A) was purchased from Eisai China Inc. (Suzhou, Jiangsu, China) and prepared to a mixed suspension at the concentration of 0.1 mg/mL. Aβ_25–35 (Lot: Y-0044) was purchased from BIOSS Biotech Limited Company (Beijing, China). Streptavidin-biotin complex (SABC) and TUNEL apoptosis detection kits were purchased from BOSTER Biotech Limited Company (Wuhan, Hubei, China). An RT-PCR kit was purchased from TransGen Biotech Limited Company (Beijing, China). Anti-Bcl-2, anti-Bax, and anti-β-actin antibodies were purchased from Bio-Rad Laboratories, Inc. (Berkeley, CA, USA). The MT-2000 Morris water maze was purchased from Chengdu Taimeng Technology Co., Ltd. (Chengdu, Sichuan, China). A ZH-LAN-STAR C type brain stereotaxic apparatus was purchased from Huaibei Zhenhua Biological Equipment Co., Ltd. (Huaibei, Anhui, China). An inverted microscope S70 was purchased from Leica DMIRB (Lognes, France). A ChemiDocXRS gel imaging and analysis system was purchased from Bio-Rad Laboratories, Inc. (Berkeley, CA, USA). An SP-9000 immunohistochemistry kit was purchased from Beijing ZSGB Biological Engineering Co., Ltd. (Beijing, China). A TC-512 PCR amplification instrument was purchased from Techen (London, UK).

Donepezil hydrochloride tablets (lot: 110822A) were purchased from Eisai China and prepared in a mixed suspension at 0.1 mg/ml. Aβ25‐35 (lot: Y‐0044) was obtained from Bioss Biotech. Streptavidin–biotin complex and terminal deoxynucleotidyl transferase dUTP nick‐end labeling (TUNEL) apoptosis detection kits were purchased from Boster Biotech. A real‐time reverse transcription–quantitative polymerase chain reaction (RT‐qPCR) kit was obtained from TransGen Biotech. Antibodies against Bcl‐2 Bax, and β‐actin were purchased from Bio‐Rad Laboratories. A brain stereotaxic apparatus (ZH‐LAN‐STAR, C type) was obtained from Huaibei Zhenhua Biological Equipment. An inverted microscope (S70) was purchased from Leica. A ChemiDoc XRS gel imaging and analysis system was obtained from Bio‐Rad Laboratories. An SP‐9000 immunohistochemistry kit was purchased from Beijing ZSGB Biological Engineering. PCR amplification instrument (TC‐512) was from Techen. Detection kits for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and ACHE were purchased from Nanjing Jiancheng Bioengineering Research Institute. Rabbit anti‐GFAP antibody (lot: 16825–1‐AP) was obtained from Proteintech. Rabbit anti‐IBA1 antibody (lot: bs‐1363R) was from Bioss Biotech.

SLF was prepared at a concentration of 100 mg/mL at the School of Pharmacy, Southwest Medical University (Luzhou, Sichuan, China) using a 1.0 g suspension equivalent to 31.5 g crude material. After purchasing donepezil hydrochloride (lot: 110823A) from Eisai Inc. (Suzhou, Jiangsu, China), a 0.1 mg/mL concentration was prepared. Aβ_25-35 (lot: Y-0044) was acquired from BIOSS Biotech Co., Ltd. (Beijing, China). Anti-AKT and anti-GSK-3β antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-tau antibody was obtained from American Bioworld Co., Ltd. (Dublin, OH, USA). Anti-GAPDH antibody was purchased from Kangchen Bio-tech Inc. (Shanghai, China). The brain stereotaxic apparatus was purchased from Huaibei Zhenhua Biological Equipment Co., Ltd. (Anhui, Huaibei, China). The Morris water maze was purchased from Chengdu Taimeng Technology Co., Ltd. (Chengdu, Sichuan, China). An S70 Inverted microscope was purchased from Leica DMIRB (Rueil-Malmaison, France). The ChemiDocXRS gel imaging and analysis system was purchased from Bio-Rad Laboratories, Inc. (Berkeley, CA, USA). SP-9000 immunohistochemistry kit was purchased from Beijing ZSGB Biological Engineering Co., Ltd. (Beijing, China). ECL Western detection reagents were from 4A Biotech Co., Ltd. (Beijing, China).