The methanol extracts of V. volvacea, T. gibbosa, T. elegans, and S. commune were investigated using TLC described by Marica et al. [45 ]. Three hundred milligrams (300 mg) of each extract was dissolved in 2 mL chloroform in a beaker and applied as spots with the aid of capillary tubes on a silica-gel coated plate (Qingdao Marine Chemical Plant, Qingdao, China) about 1 cm from the base. The spotted plates were run in a developed solvent system of 100% chloroform. The developed plate was observed under ultra-violet light using both short and long wavelengths (254 and 365 nm) and then sprayed with anisaldehyde (Sigma-Aldrich, London, UK) in order to reveal compounds present in the extracts. Distances between the spots were measured and the retention factor (Rf) values were calculated, using the following:
Anisaldehyde
Manufactured by Merck Group
Sourced in Germany
Anisaldehyde is a chemical compound used in various laboratory applications. It is a clear, colorless liquid with a characteristic floral aroma. Anisaldehyde is commonly utilized as a reagent in organic synthesis, analytical chemistry, and other scientific research. Its core function is to serve as a versatile chemical tool for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
Sulfuric acid, by Merck Group (4 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Toluene, by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Polyethylene glycol peg, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Peg400, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Methanol, by Avantor (1 mentions)
Chloroform, by Merck Group (1 mentions)
Lithium chloride (licl), by Merck Group (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
11 protocols using anisaldehyde
1
Thin Layer Chromatography of Fungal Extracts
2
Qualitative Analysis of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethanol, ethyl acetate, toluene and formic acid were purchased from Merck (KGaA, Darmstadt, Germany). Polyethylene glycol (PEG) and anisaldehyde were from Sigma-Aldrich (Steinheim am Albuch, Baden-Württemberg, Germany). Additionally, 2-aminoethyl diphenylborinate (NTS) was purchased from Fluka (Steinheim am Albuch, Baden-Württemberg, Germany). All solvents used for extraction, for mobile phase preparation and plate derivatization were of analytical purity grade.
3
HPTLC Analysis of Eupatorin and Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anisaldehyde (Ref.: A88107), diphenylboric acid aminoethyl ester (Ref.: 42810), PEG 400 (Ref.: P3265), and rutin (Ref.: 78095) were obtained from Sigma Chemical Company (St. Louis, MO, USA). Eupatorin was from our own laboratory collection (previously isolated from Salvia officinalis subsp. lavandulifolia leaves). Acetic acid, formic acid, sulfuric acid, dichloromethane, ethanol, ethyl acetate, methanol, and toluene were of analytical grade and purchased from Panreac Química (Barcelona, Spain). HPTLC glass plates coated with Silicagel 60F254 (20 × 10 cm) were purchased from Merck (Darmstadt, Germany; Ref.: 1.05642.0001).
4
Purification and Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol (p.a.) was purchased from VWR International AB. Ethanol (>99.5%) was purchased from Solveco AB. Toluene (HPLC grade, >99.8%) was purchased from RCI Labscan. Chloroform (≥99.8%), lithium chloride (≥99%), potassium hydroxide, sulfuric acid (95–97%), acetic acid glacial (100%), dichloromethane (anhydrous, stabilized with amylene, ≥99.8%), acetic anhydride (p.a., >99.5%), pyridine (>99.5%), anisaldehyde (4-methoxybenzaldehyde, for synthesis), and resorcinol were purchased from Sigma-Aldrich, Merck KGaA (Darmstadt, Germany). Silica gel S (particle size: 32–63 μm, 230–400 mesh ASTM) were purchased from Riedel-de Haën. DEAE-cellulose 23 was purchased from Whatman. Polyisobutylmethacrylate was purchased from Sigma-Aldrich. Deionized water (Milli Q) was prepared with Purelab Flex 2 water purification system (AB Ninolab) and all organic solvents were redistilled prior to use.
5
Profiling Fermented Bamboo Shoots Volatiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Several chemical standards were purchased from Sigma-Aldrich (Shanghai, China): propanal, ethyl acetate, octanal, nonanal, 1-octen-3-ol, (E)-2-octenal, acetic acid, benzaldehyde, 1-nonanol, methyl salicylate, guaiacol, geranylacetone, phenylethyl alcohol, creosol, 4-ethylguaiacol, anisaldehyde, p-cresol, and 4-ethyl-phenol. The internal standard (2-methyl-3-heptanone) and a C7-C40 n-alkane mixture were purchased from Sigma-Aldrich. Chemical standards and internal standards were of a high-purity grade (GC grade ≥ 97% purity). GFBS were collected from several fermentation workshops (in the City of Liuzhou (LZ), Nanning (NN), Guilin (GL), and Baise (BS), Guangxi Zhuang Autonomous Region, China), in which the fresh shoots of Dendrocalamus latiflorus Munro were peeled and then steeped in mountain spring water for anaerobically fermenting in a jar for 30 d at 25 °C. Their basic information is listed in Appendix A Table A1 . All samples were wrapped in nylon/polyethylene and stored at −20 °C until analysis.
6
Bioactivity Measurements for Natural Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The bioactivity measurements were performed on a 96-well microplate reader (Perkin Elmer Multimode Plate Reader EnSpire) at the National Center of Biotechnology Research. Gallic acid, Folin-Ciocalteu's Reagent (FCR), aluminum trichloride, 1,1'-diphenyl-2-picrylhydrazyl (DPPH), butylatedhydroxyltoluene (BHT), ascorbic acid, α-tocopherol, butylatedhydroxylanisole (BHA), dimethyl sulfoxyde (DMSO), diammonium salt (ABTS), nitro blue tetrazolium (NTB), neocuproine 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonicacid), butyrlcholinesterase from horse serum (BChE, EC 3.1.1.8, 7.8 U/mg, Sigma), acetylcholinesterase from electric eel (AChE, Type-VI-S, EC 3.1.1.7, 827.84 U/mg, Sigma), acetylthiocholine iodide, S-butyrylthiocholine iodide, galantamine, 5,5′-dithiobis (2-nitrobenzoic) acid (DTNB), anis aldehyde, sulfuric acid, and glacial acetic acid were obtained from Sigma Aldrich (Stern-heim, Germany). Potassium persulfate, copper (II) chloride and sodium carbonate were obtained from Biochem Chemopharma. All other chemicals and solvents were of analytical grade . Antibiotics were obtained from Titan Media, India.
7
Phytochemical Profiling of Officinalis Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The used reference compounds chlorogenic acid (100% purity), luteolin-7-glucoside (99% purity), luteolin (98% purity), quercetin (100% purity), caffeic acid (100% purity), hyperoside (100% purity), rutin (100% purity), vitexin (95% purity), oleanolic acid (purity 100%) were pharmacopoeial reference standards of the State Pharmacopoeia of Ukraine. Reference compounds (isorhamnetin-3-O-(2′′,6′′-di-rhamnosyl)-glucoside, isorhamnetin-3-O-rutinoside)) were obtained previously from C. officinalis (Derkach 1989) .
The solvents (ethanol, methanol, toluene, butanol, acetonitrile, water, and ethyl acetate) and chemicals (anhydrous acetic acid, oxalic acid, sulfuric acid, anhydrous formic acid, boric acid, and anisaldehyde) used in the experiments were of analytical grade and were purchased from Sigma-Aldrich (Germany) and Merck (Darmstadt, Germany).
The solvents (ethanol, methanol, toluene, butanol, acetonitrile, water, and ethyl acetate) and chemicals (anhydrous acetic acid, oxalic acid, sulfuric acid, anhydrous formic acid, boric acid, and anisaldehyde) used in the experiments were of analytical grade and were purchased from Sigma-Aldrich (Germany) and Merck (Darmstadt, Germany).
8
Analytical Techniques for Chemical Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All used chemicals were of analytical grade. Dichloromethane, toluene, ethanol, methanol, ethyl acetate, glacial acetic acid, 95% sulfuric acid and anhydrous magnesium sulfate were purchased from POCH S.A. (Gliwice, Poland). Anisaldehyde, the homologous series of n-alkanes C 9 -C 25 , and all compounds used as references substances were purchased from Sigma-Aldrich (Poznań, Poland). Strata SDB-L SPE cartridges were purchased from Phenomenex and silica gel 60 HPTLC aluminum plates (20 cm × 10 cm) were purchased from Merck.
9
Synthesis and Characterization of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anisaldehyde, 3,5-dichloroaniline, and monohydrated hydrazine were purchased from Merck (Darmstadt, Germany), phenol, ascorbic acid, urease (from jack bean), DPPH (2,2-diphenyl-1-picrylhydrazyl), acetylthiocholine iodide (ATCI), acetylcholinesterase, and 5,5-ditiobis(2-nitrobenzoic) acid (DTNB) were from Sigma-Aldrich (Steinheim, Germany). Sodium hypochlorite, and sodium salicylate were from Daejung (Siheung City, Korea). Lithium chloride, sodium nitrite and sodium nitroprusside were from Riedel de Haën (Seelze, Germany). Urea, ethylenediaminetetraacetic acid and thiourea were from BDH Labs (Cambridge, England).
10
Quantitative Analysis of Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gallic acid, oleanolic acid, diosgenin, alpha-linolenic acid, linoleic acid and oleic acid (as reference standards) and Folin–Ciocalteu reagent, vanillin, perchloric acid, sulfuric acid, dimethyl sulfoxide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate, acetic acid, hydrochloric acid and anisaldehyde were obtained from Merck KGaA (Darmstadt, Germany). All other solvents and chemicals used were of analytical or HPLC grade. The working solutions were prepared immediately prior to measurement.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!