Log In Sign up
The largest database of trusted experimental protocols

Negative selection microbeads kit

Manufactured by Miltenyi Biotec
Visit Supplier

The Negative selection microbeads kit is a laboratory product designed for the isolation of specific cell types from complex mixtures. The kit contains magnetic beads coated with antibodies that bind to unwanted cells, allowing the desired cells to be separated from the sample through magnetic separation.

Automatically generated - may contain errors

Lab products found in correlation

Facsmelody, by BD (4 mentions) Cd4 okt4 bv510, by BioLegend (2 mentions) Cd8 rpa t8 fitc, by BioLegend (2 mentions) Cd3 percp ucht1, by BioLegend (2 mentions) H 2 db asnenme apc, by Immudex (1 mentions) H 2 db sslenfrayv pe, by Immudex (1 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Negative selection (51 citations) Negative selection kit (47 citations) MicroBead kits (12 citations) Negative selection microbeads (2 citations) Negative selection magnetic microbeads kit (2 citations)

4 protocols using negative selection microbeads kit

1

CD8+ T Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD8+ T cells were isolated from PBMCs using a negative-selection microbeads kit (Miltenyi Biotec) according to the manufacturer’s protocol. Next, CD8+ T cells were labeled at room temperature for 20 min with H-2 Db ASNENMETM-APC and H-2 Db SSLENFRAYV-PE (Immudex, Virum, Denmark). Next, surface staining was performed using the following mAbs: CD3(17A2)FITC, CD4(GK1.5)- BrilliantViolet510, and CD8(53.6.7)-BrilliantViolet786 (all BD). CD3+CD4−CD8+dextramer+ cells were then sorted directly into RNAlater (Ambion Inc. Applied Biosystems) using a FACS Melody (BD) and subsequently stored at −80 °C for TCRβ clonotype analysis.
Lanfermeijer J., van de Ven K., Hendriks M., van Dijken H., Lenz S., Vos M., Borghans J.A., van Baarle D, & de Jonge J. (2024). The Memory-CD8+-T-Cell Response to Conserved Influenza Virus Epitopes in Mice Is Not Influenced by Time Since Previous Infection. Vaccines, 12(4), 419.
+ Open protocol
+ Expand
2

Isolation and Characterization of Antigen-Specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD8 + T cells were isolated from PBMCs using a negative selection microbeads kit (Miltenyi Biotec) according to the manufacturer’s protocol. CD8 + T cells were subsequently labeled at room temperature for 20 min with the A*0201/GILG dextramer (PE, Immudex) and corresponding dextramers manufactured with the modified peptides (APC, Immudex). Subsequently, surface staining was performed using the following mAbs: CD3(17A2)-FITC (cat. Nr. 555274, diluted 1:100), CD4(GK1.5)-BV510 (cat. Nr. 743155, diluted 1:800), CD8(53–6.7)-BV786 (cat. Nr. 563332, diluted 1:400) (All BD Biosciences). CD3 + CD4 − CD8+dextramer+ cells were then sorted using a FACS Melody (BD) directly into RNAlater (Ambion Inc. Applied Biosystems) and stored at −80 °C for subsequent TCRβ clonotype analysis.
Lanfermeijer J., van de Ven K., van Dijken H., Hendriks M., Talavera Ormeño C.M., de Heij F., Roholl P., Borghans J.A., van Baarle D, & de Jonge J. (2023). Modified influenza M158–66 peptide vaccination induces non-relevant T-cells and may enhance pathology after challenge. NPJ Vaccines, 8, 116.
+ Open protocol
+ Expand
3

Isolating and Characterizing CMV-Specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD8+ T cells were isolated from PBMCs using a negative selection microbeads kit (Miltenyi Biotec) according to the manufacturer's protocol. Next, CD8+ T cells were labeled at room temperature for 20 min with the A*0201/GLCTLVAML-APC dextramer and with the A*0201/NLVPMVATV-APC dextramer for CMV+ individuals. Subsequently surface staining was performed using the following mAbs: CD3(UCHT1)-PerCP (Biolegend), CD4(OKT4)-BV510 (Biolegend), and CD8(RPA-T8)-FITC (Biolegend). CD3+CD4−CD8+dextramer+ cells were then sorted by FACS Melody (BD) directly into RNAlater (Ambion Inc. Applied Biosystems) and stored at −80°C for subsequent TCRβ clonotype analysis.
Lanfermeijer J., de Greef P.C., Hendriks M., Vos M., van Beek J., Borghans J.A, & van Baarle D. (2021). Age and CMV-Infection Jointly Affect the EBV-Specific CD8+ T-Cell Repertoire. Frontiers in Aging, 2, 665637.
+ Open protocol
+ Expand
4

Isolation and Characterization of GILG-Specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD8+ T cells were isolated from PBMCs using a negative selection microbeads kit (Miltenyi Biotec). Next, CD8+ T cells were labeled at room temperature for 20 minutes with the A*0201/GILGFVFTL-APC dextramer (Immudex) (GILG). Subsequently surface staining was added with the following mAbs: CD3(UCHT1)-PerCP (Biolegend), CD4(OKT4)-BV510 (Biolegend) and CD8(RPA-T8)-FITC (Biolegend) and CD3+CD4-CD8+GILG+ cells were sorted by FACS Melody (BD) directly into RNAlater (Ambion Inc. Applied Biosystems) and stored at -80°C for subsequent TCRβ clonotype analysis.
van den Berg S.P., Lanfermeijer J., Jacobi R.H., Hendriks M., Vos M., van Schuijlenburg R., Nanlohy N.M., Borghans J.A., van Beek J., van Baarle D, & de Wit J. (2021). Latent CMV Infection Is Associated With Lower Influenza Virus-Specific Memory T-Cell Frequencies, but Not With an Impaired T-Cell Response to Acute Influenza Virus Infection. Frontiers in Immunology, 12, 663664.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!