Plasma glucose was measured in the laboratory of Turku PET Centre in duplicate using the glucose oxidase technique (Analox GM7 or GM9, Analox Instruments Ltd., London, UK). Glycosylated haemoglobin (HbA1c) and plasma insulin were measured with automated electrochemiluminescence immunoassay (Cobas 8000; Roche Diagnostics, Mannheim, Germany).
Analox gm7
Manufactured by Analox Instruments
Sourced in United Kingdom
The Analox GM7 is a compact, digital gas monitor designed for continuous monitoring of gases in a variety of industrial and laboratory applications. The device provides real-time measurement of gas concentrations and can be configured to detect multiple gas types simultaneously. The GM7 features a clear digital display and offers various output options for integration with other systems.
Automatically generated - may contain errors
Lab products found in correlation
Autodelfia, by PerkinElmer (4 mentions)
Cobas 8000, by Roche (3 mentions)
Acs acod, by Fujifilm (2 mentions)
Is130d, by Calbiotech (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Variant 2 haemoglobin a1c, by Bio-Rad (1 mentions)
Modular p800 automatic analyzer, by Roche (1 mentions)
Modular p800 automatic analyser, by Roche (1 mentions)
Creatinine, by Merck Group (1 mentions)
Leptin, by Merck Group (1 mentions)
9 protocols using analox gm7
1
Plasma Glucose and HbA1c Analysis
2
Euglycemic-Hyperinsulinemic Clamp Glucose Measurements
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Plasma glucose during the euglycemic–hyperinsulinemic clamp was determined in duplicate by the glucose oxidase method (Analox GM7 or GM9, Analox Instruments, London, UK). Serum insulin concentration, determined every 30 min during the clamp, was measured by a double antibody RIA (Phadeseph Insulin RIA kit, Pharmacia & Upjohn, Uppsala, Sweden) or automatized electro-chemiluminescence immunoassay (Cobas 8000, Roche Diagnostics GmbH), and serum FFA concentration, determined every 60 min during the clamp was measured using an enzymatic assay (ACS-ACOD, Wako Chemicals GmbH, Neuss, Germany).
3
Plasma Glucose and Insulin Assay
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Plasma glucose was assessed using the glucose oxidase method via an automated analyzer (Analox GM7, Analox Instruments Ltd., Lunenburg, MA, USA). Serum insulin concentrations were determined via commercially available enzyme-linked immunosorbent assay kits (IS130D; Calbiotech, Inc., Spring Valley, CA, USA) and a spectrophotometer (Eon, BioTek Instruments, Inc., Winooski, VT, USA). Following analyses, total area under the glucose and insulin curves (AUCglu and AUCins, respectively) were calculated using a standard trapezoidal method. To eliminate interassay variance, all samples were analyzed in duplicate by a single technician. The coefficient of variation for each assay was 0.87% for glucose and 8.24% for insulin.
4
Endometrial Glucose Consumption Assay
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To examine glucose consumption, endometrial organ cultures or endometrial cells were treated in RPMI-1640 medium (cataologue number R8758, Sigma) with control vehicle or LPS (100 ng/ml), and supernatants collected periodically to measure the concentration of glucose. The concentration of glucose was measured using a clinical chemistry analyzer (Analox GM7; Analox Instruments, London, UK) according to the manufacturer’s instructions. Briefly, the device was calibrated using a glucose standard before 10 μl samples were measured in duplicate. The intra-assay coefficient of variation was 1.4%.
5
Comprehensive Metabolic Biomarker Assessment
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Plasma glucose was measured in the laboratory of Turku PET Centre in duplicate using the glucose oxidase technique (Analox GM7 or GM9, Analox Instruments Ltd., London, UK). Glycosylated hemoglobin (HbA1c) was measured with ion-exchange high performance liquid chromatography (Variant II Haemoglobin A1c, Bio-Rad Laboratories, CA, USA). Plasma insulin was determined by time-resolved immunofluorometric assay (AutoDELFIA, Perkin Elmer Inc, Wallac Oy, Turku, Finland). Serum FFA were measured with a photometric enzymatic assay (Wako Chemicals GmbH, Neuss, Germany) on Modular P800 automatic analyzer (Roche Diagnostics, Mannheim, Germany). Serum high-sensitivity C-reactive protein (hs-CRP) was analysed with the sandwich immunoassay method using an Innotrac Aio1 immunoanalyzer (Innotrac Diagnostics, Turku Finland). Serum adipokines were analysed in duplicate by using Milliplex Human Serum Adipokine (Panel A) kit [cat.no HADK1-61K-A] (MilliporeSigma, Burlington, MA, USA) containing interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNFα), and leptin.
6
Metabolic Biomarker Measurement Protocol
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Plasma glucose was measured in the laboratory of the Turku PET Centre in duplicate using the glucose oxidase technique (Analox GM7 or GM9, Analox Instruments, London, UK). HbA1c was measured with ion‐exchange high performance liquid chromatography (Variant II HbA1c, Bio‐Rad Laboratories, CA, USA). Serum insulin was determined by time‐resolved immunofluorometric assay (AutoDELFIA, Perkin Elmer Life and Analytical Sciences). Serum FFA were measured with a photometric enzymatic assay (Wako Chemicals, Neuss, Germany) on a Modular P800 automatic analyser (Roche Diagnostics, Mannheim, Germany). Serum high‐sensitivity C‐reactive protein was analysed by the sandwich immunoassay method using an Innotrac Aio1 immunoanalyser (Innotrac Diagnostics, Turku, Finland). Serum adipokines were analysed in duplicate by using Milliplex Human Serum Adipokine (Panel A) kit (cat. no. HADK1‐61 K‐A) containing interleukin‐6 (IL‐6), IL‐8, tumour necrosis factor alpha and leptin.
7
Quantification of Metabolic Markers
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Plasma glucose was determined in duplicate by the glucose oxidase method (Analox GM7 or GM9, Analox Instruments, London, UK). Serum insulin concentration was measured by a double antibody radioimmunoassay (Phadeseph Insulin RIA kit, Pharmacia & Upjohn, Uppsala, Sweden), fluoroimmunometric assay (AutoDELFIA, PerkinElmer Inc, Turku, Finland) or automatized electro-chemiluminescence immunoassay (Cobas 8000, Roche Diagnostics GmbH, Mannheim, Germany). Alanine aminotransferase and aspartate aminotransferase were measured using automatized enzymatic method (Cobas 8000, Roche Diagnostics GmbH, Mannheim, Germany) and serum FFA concentration using an enzymatic assay (ACS-ACOD, Wako Chemicals GmbH, Neuss, Germany).
8
Bone Biomarker Measurement Methods
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Plasma concentration of glucose was measured using the glucose oxidase method (Analox GM7 or GM9; Analox Instruments Ltd., London, UK) and serum insulin was determined by time-resolved immunofluorometric assay (AutoDELFIA; PerkinElmer Life and Analytical Sciences , Waltham, MA). Bone resorption was assessed by C-terminal crosslinked telopeptides of type I collagen (CTX, IDS-iSYS CTX-I ELISA, CrossLaps®) and bone formation by intact N-terminal propeptide of type I collagen (PINP, IDS-iSYS Intact PINP assay), both from IDS Ltd., East Boldon, UK. Serum total osteocalcin was determined with 2-site immunoassay based on monoclonal antibodies 2H9 and 6F9 using previously described protocol (23 (link)).
9
Fasting Metabolic Markers Assessment
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Fasting plasma glucose (FPG) and triglycerides (TGs) were measured using an Analox GM7 analyzer (Analox Instruments, London, UK). Fasting plasma non-esterified fatty acids (NEFA) (WAKO, Osaka, Japan), creatinine (Sigma-Aldrich), insulin (FPI), and leptin (EMD Millipore) were measured using commercially available kits. All samples were analyzed in duplicate and run in a single assay with intra-assay coefficients of variability of < 10%. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as follows: HOMA-IR = (FPG × FPI)/2.430, with FPG in milligrams per deciliter and FPI in microunits per milliliter, as previously described [34] .
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