Pi3k c2α

SEVO and ISO were purchased from Dainabot Company Limited (Osaka, Japan). Polyclonal antibodies against phospho-MLC (Thr18/Ser19), phospho-MYPT1 (Thr853), phospho-MYPT1 (Thr696), phospho-CPI-17 (Thr38), MLC2 (FL-172), MYPT1 (H-130), CPI-17 (H60), PI3K-p85α, and Rock-II, and the secondary antibody labeled with horseradish peroxidase (HRP) were all obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). PI3K-C2α was purchased from Abcam, Inc. (Tokyo, Japan). LY294002 was provided from Merck-Calbiochem Biosciences (Darmstadt, Germany). Y27632 was purchased from Calbiochem-Novabiochem Corporation (San Diego, CA, USA). All other reagents for experiments and western blotting were of analytical grade.

Western blotting was performed as previously described [17 (link)]. Primary antibodies against KIF15, FAK, FAK-397, PXN, PXN-118, SRC, SRC-418, RAB11A, PI3K-C2α, and FLAG were purchased from Abcam. The Integrin Antibody Sampler Kit and antibody against GAPDH were purchased from Cell Signaling Technology. HRP-conjugated secondary antibodies, including polyclonal goat mouse IgG and polyclonal goat rabbit IgG, were purchased from Cell Signaling. GADPH was used as a normalization loading control. Expression levels were quantified by Imaging Systems (Thermo Fisher), and the relative expression values were quantified using Image J software.