Anti rara

Manufactured by Santa Cruz Biotechnology

Anti-RARA is a laboratory reagent that targets the retinoic acid receptor alpha (RARA) protein. RARA is a nuclear receptor that plays a role in regulating gene expression. Anti-RARA can be used in various research applications to study the function and regulation of RARA.

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Lab products found in correlation

Anti npm antibody, by Thermo Fisher Scientific (1 mentions) Anti parp, by Cell Signaling Technology (1 mentions) Ab8895, by Abcam (1 mentions) Ab41902, by Abcam (1 mentions) Ab14984, by Abcam (1 mentions) Ab24552, by Abcam (1 mentions) Ab64644, by Abcam (1 mentions) Nb100 2220, by Novus Biologicals (1 mentions) To901317, by Merck Group (1 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (1 mentions) Anti β actin sc 1616, by Santa Cruz Biotechnology (1 mentions) Anti atf3, by Santa Cruz Biotechnology (1 mentions) Anti atf5, by Santa Cruz Biotechnology (1 mentions) Bax inhibiting peptide v5, by Merck Group (1 mentions) Auto histone chip seq kit protein a, by Diagenode (1 mentions) Ab41928, by Abcam (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Anti gfp, by Transgene (1 mentions) Anti p62, by Santa Cruz Biotechnology (1 mentions) Anti ago2, by Abnova (1 mentions)

4 protocols using anti rara

Antibody Preparation for Cell Signaling

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Rabbit polyclonal anti-RARA and anti-TRADD antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse monoclonal anti-NPM antibody was obtained from Invitrogen (Carlsbad, CA). Anti-PARP was obtained from Cell Signaling Technology (Danvers, MA), and anti-caspase-3 was from Assay Designs (Ann Arbor, MI). Anti-β-actin and anti-FLAG mouse monoclonal antibodies were purchased from Sigma (St. Louis, MO).

Chattopadhyay A., Hood B.L., Conrads T.P, & Redner R.L. (2014). Extrinsic Apoptosis is Impeded by Direct Binding of the APL Fusion Protein NPM-RAR to TRADD. Molecular cancer research : MCR, 12(9), 1283-1291.

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Analyzing Transcriptional Regulators in Mycobacterial Infection

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Anti-LXRα (ab41902 Abcam), anti-ATF1 (sc270, Santa Cruz), anti-ATF3 (sc188, Santa Cruz), anti-ATF5 (sc377168, Santa Cruz), anti-RXRA (sc553, Santa Cruz), anti-RARA (sc551, Santa Cruz), anti-MEF2A (ab7606. Abcam), anti-MEF2C (ab64644, Abcam), anti-LC3 (NB100-2220, Novus), anti-β-actin (sc-1616, Santa Cruz), anti-H3K27ac (ab4729),anti-NCoR (ab24552), anti-KAT3B/p300 (ab14984), anti-KAT13A/SRC1/NCoA1 (ab84), anti-H3K4me1 (ab8895, abcam), anti-PPARγ (ab41928, Abcam), anti-Mtb-FITC (ab20962, Abcam), anti-rabbit Alexa647 (A-21245, Life Technologies), Prolong Gold with Dapi (Life Technologies), anti-rabbit-Alexa488 (Life Technologies), Bodipy 493 (Life Technologies). TO901317, ATRA and BAX Inhibiting Peptide V5 were bought from Sigma and resupended in DMSO. Nelfinavir (Nel) and Ritonavir (Rit) were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases. Auto Histone ChIP-seq Kit (Protein A) was bought from Diagenode.

Bouttier M., Laperriere D., Memari B., Mangiapane J., Fiore A., Mitchell E., Verway M., Behr M.A., Sladek R., Barreiro L.B., Mader S, & White J.H. (2016). Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Research, 44(22), 10571-10587.

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Western Blot Protein Detection

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Protein extracts were boiled in RIPA buffer (Beyotime, Shanghai, China) and separated in a SDS–polyacrylamide electrophoresis gel. The proteins were then transferred to a polyvinylidene fluoride membrane (Millipore) and probed with anti-RARA (Santa Cruz), anti-AGO2 (Abnova, Taipei, Taiwan), anti-p62 (Santa Cruz), anti-GABARAP (Proteintech, Chicago, IL, USA), anti-GFP (TransGen Biotech), anti-ULK1 (Novus Biologicals, Littleton, CO, USA), anti-LC3B (Novus Biologicals), anti-DRAM2 (Sigma-Aldrich), anti-E2F1 (Millipore) and anti-GAPDH (Sigma-Aldrich) antibodies.

Chen Z.H., Wang W.T., Huang W., Fang K., Sun Y.M., Liu S.R., Luo X.Q, & Chen Y.Q. (2016). The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway. Cell Death and Differentiation, 24(2), 212-224.

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NPM-RAR Protein Expression and Characterization

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Rabbit polyclonal anti-RARA and rabbit polyclonal anti-p65 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-β-actin and anti-FLAG mouse monoclonal antibodies were purchased from Sigma (St. Louis, MO). Anti-lamin and anti-V5 antibodies were purchased from Invitrogen (Gran Island, NY). Anti-hsp90 antibody was obtained from Enzo Life Sciences (Farmingdale, NY). Human recombinant TNF-alpha was purchased form R&D Systems (Minneapolis, MN). NPM-RAR expression vector was generated by subcloning the NPM-RAR coding sequence into the multiple cloning site of pSG5 (Agilent Technologies, Santa Clara, CA). The coding region of TRADD was subcloned into pcDNA3/V5-His (Invitrogen) in the appropriate reading frame to generate fusion proteins bearing a V5-His tag at the C terminus. Fidelity was confirmed by DNA sequencing and Western blotting.

Chattopadhyay A., Abecassis I, & Redner R.L. (2015). NPM-RAR binding to TRADD selectively inhibits caspase activation, while allowing activation of NFκB and JNK. Leukemia & lymphoma, 56(12), 3401-3406.

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