Body weight and height were measured with the participants wearing light indoor clothing and no shoes, and BMI (kg/m2) was calculated. Systolic BP (SBP) and diastolic BP (DBP) measurements were obtained from the participants in a sitting position after a 10 min resting period. Blood samples were collected from patients following at least 8 h of fasting. TG, HDL-C, and fasting plasma glucose (FPG) were measured using the AU5822 automated clinical analyzer (Beckman Coulter, Brea, CA, USA).
Au5822
Manufactured by Beckman Coulter
Sourced in United States
The AU5822 is a fully automated chemistry analyzer designed for high-volume clinical laboratories. It offers a compact and efficient solution for performing a wide range of routine clinical chemistry tests.
Automatically generated - may contain errors
13 protocols using au5822
1
Anthropometric and Metabolic Measurements
2
Comprehensive Blood Biomarker Analysis
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Whole blood was collected in clot activator tube, centrifuged at 1500× g for 15 min at 4 °C to obtain the serum, and analyzed within 48 h. Serum lipid profile (total cholesterol (TC)), TG, LDL and HDL, liver function (total protein, albumin, globulin, AG ratio), AST, ALT, ALP, total bilirubin, and renal function (sodium, urea, chloride, potassium, creatinine, uric acid, calcium, and phosphate) profile were performed in a MS ISO 15189 certified Advanced Diagnostic laboratory using an autoanalyzer AU5822 (Beckman Coulter, Brea, CA, USA) according to the method recommended by International Federation for Clinical Chemistry (IFCC).
3
Biomarker Kinetics After Intense Exercise
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Each blood sample was collected venously via venipuncture and filled into 10 mL serum vacutainers. These were centrifuged at 2500 rpm for 15 min, and the resulting supernatant was pipetted into 2 mL Eppendorf tubes and immediately stored in a refrigerator at -32°C. For analysis purposes, the frozen tubes were transported to a local laboratory and analyzed separately. The Beckman Coulter AU5822 (23 (link)) was used for detecting creatine kinase (CK), lactate dehydrogenase (LDH) and myoglobin (MYO). High-sensitivity C-reactive protein (hsCRP) was detected “pre” & “24 h” by immunoturbidimetry (Alinity c CRP Vario Reagent Kit, Abbott Laboratories) with a coefficient of variance (CV) of 12.5%. The “24 h” measurement was chosen due to the high likelihood of CRP levels peaking 1 day after intense exercise (24 (link), 25 (link)). As some participants were unable to provide blood at the given times, they were excluded from the data analysis.
4
Measuring Serum Enzyme Activities
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Rat serum amylase and lipase activity were measured using an AU 5822 automated chemistry analyzer (Beckman Coulter, Brea, Calif). Amylase and trypsin activities in the supernatant of AR42J cells were evaluated using assay kits purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
5
EHR-Derived Patient Data Analysis
6
Plasma CK Measurement Post-Exercise
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Plasma CK collected pre and immediately post exercise and 24, 48 and 72 h post exercise was measured at a commercial laboratory (Canterbury Health Laboratories, Christchurch, New Zealand). The analysis of CK was quantified by spectrophotometry, which measured the rate of nicotinamide adenine dinucleotide (NADH) formation from an enzymatic reaction using an automated Beckman Coulter AU5822 analyser. Plasma, and not serum, was the preferred analyte by the laboratory, and the reference intervals for plasma CK in males were reported to be between 60 and 22 U/L.
7
Sodium Measurement Protocols Comparison
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Basic metabolic panel (BMP) sodium measurements were performed on a Vitros 5600 or Vitros 4600 chemistry analyzer, as well as a Beckman Coulter AU 5822 (Beckman Coulter, Brea, CA). Blood gas (BG) sodium measurements were obtained on Siemens RAPIDLab 1265 BG analyzers (Siemens, Tarrytown, NY) in the central laboratory and by iSTAT (Abbott Point of Care, Abbott, Lake Bluff, IL) at the bedside, using the manufacturer’s reagents and parameters.
8
Cytokine and Metabolite Profiling in Psoriasis
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IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, and IFN-γ were quantified in sera from healthy controls and subjects with psoriasis by Th1/Th2/Th17 cytokine assay (JiangXi Cellgene Biotech Co., LTD, China) according to the manufacturers' instructions. Data were acquired using a Navios Cytometer (Beckman Coulter Company). Standard curves were constructed, and calculations were performed using JiangXi Cellgene Biotech Co., LTD CBA software.
Arg-1 was quantified in sera from healthy controls and subjects with psoriasis by a quantitative colorimetric arginase determination assay (Quanti Chrom Arginase Assay Kit, DARG-200, Bioassay Systems) according to the manufacturer's instructions.
NO was quantified in sera from healthy controls and subjects with psoriasis using the NO kit (Moledia Technology Corp. of Beijing) and AU5822 (Beckman Coulter), according to the manufacturer's instructions. Serum iNOS level was quantified using iNOS Detection kits (A014-1, Nanjing Jiancheng Bioengineering Institute) according to the manufacturer's instructions.
Arg-1 was quantified in sera from healthy controls and subjects with psoriasis by a quantitative colorimetric arginase determination assay (Quanti Chrom Arginase Assay Kit, DARG-200, Bioassay Systems) according to the manufacturer's instructions.
NO was quantified in sera from healthy controls and subjects with psoriasis using the NO kit (Moledia Technology Corp. of Beijing) and AU5822 (Beckman Coulter), according to the manufacturer's instructions. Serum iNOS level was quantified using iNOS Detection kits (A014-1, Nanjing Jiancheng Bioengineering Institute) according to the manufacturer's instructions.
9
Plasma NGAL Measurement by Turbidimetric Immunoassay
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Plasma levels of NGAL were measured using a particle-enhanced turbidimetric immunoassay (NGAL TEST™, BioPorto Diagnostics A/S, Hellerup, Denmark) on a Beckman Coulter AU 5822 (Beckman Coulter, USA) according to the manufacturer's instructions. Briefly, plasma samples from the subjects were mixed with reaction buffer and incubated for a short time. Subsequently, an immunoparticle suspension (polystyrene microparticles coated with mouse monoclonal antibodies to NGAL) were added to cause aggregation of the immunoparticles. After quantifying the degree of aggregation using a light absorption test, plasma levels of NGAL were calculated based on an established calibration curve (Lippi et al. 2011) .
10
Comprehensive Blood Biochemical Analysis
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Trunk blood was collected by cardiac puncture into clot activator tube,
K2EDTA tube, and sodium fluoride tube for subsequence analysis.
All blood samples were kept at 4°C and processed within 24hrs after
collection. The whole blood samples in clot activated tube were used for blood
serum biochemical analysis in an MS ISO 1589 certified Advance Diagnosis
laboratory (Advanced Medical and Dental Institute, USM, Malaysia). The blood
sample was centrifuged at 1,500×g at 4°C for 15 min to separate
the serum and immediately collected into a new tube. Blood serum was analyzed
within 48hrs after extraction, using an auto-analyzer AU5822 (Beckman Coulter,
Brea, CA, USA) according to the protocol recommended by International Federation
for Clinical Chemistry (IFCC). Liver function profile (albumin, total protein,
globulin, albumin/globulin ratio [A/G], alanine aminotransferase [ALT], alkaline
phosphatase [ALP] and aspartate aminotransferase [AST]), renal function profile
(calcium, chloride, creatinine, phosphate, potassium, sodium, urea and uric
acid) and fasting blood sugar was measured.
K2EDTA tube, and sodium fluoride tube for subsequence analysis.
All blood samples were kept at 4°C and processed within 24hrs after
collection. The whole blood samples in clot activated tube were used for blood
serum biochemical analysis in an MS ISO 1589 certified Advance Diagnosis
laboratory (Advanced Medical and Dental Institute, USM, Malaysia). The blood
sample was centrifuged at 1,500×g at 4°C for 15 min to separate
the serum and immediately collected into a new tube. Blood serum was analyzed
within 48hrs after extraction, using an auto-analyzer AU5822 (Beckman Coulter,
Brea, CA, USA) according to the protocol recommended by International Federation
for Clinical Chemistry (IFCC). Liver function profile (albumin, total protein,
globulin, albumin/globulin ratio [A/G], alanine aminotransferase [ALT], alkaline
phosphatase [ALP] and aspartate aminotransferase [AST]), renal function profile
(calcium, chloride, creatinine, phosphate, potassium, sodium, urea and uric
acid) and fasting blood sugar was measured.
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