Triglycerides assay kit

A total of 5 ml of venous blood samples were collected after 12 to 14 h of fasting in the morning h with minimum stasis, using evacuated collection tubes (Vacuette^®; Greiner Bio-One International GmbH, Vienna, Austria). To separate the plasma, the collected blood samples were centrifuged at 2,000 × g for 20 min at 25°C. A total of 500 µl aliquots of plasma samples were stored at −80°C. The level of total cholesterol was investigated using a Total Cholesterol assay kit (cat. no. CH200; Randox Laboratories Ltd., Antrim, UK) and the level of triglycerides was investigated using a Triglycerides assay kit (cat. no. TR210; Randox Laboratories Ltd.), and both total cholesterol and triglyceride levels were then determined using a Cobas-Fara II Clinical Chemistry Auto analyzer (Roche Diagnostics GmbH, Mannheim, Germany). For the estimation of high density lipoprotein-cholesterol (HDL-c), the non-HDL fractions were precipitated with a mixture of 2.4 mmol/l phosphotungstic acid and 39 mmol/l magnesium chloride (Bayer Diagnostics India Ltd., Baroda, India) prior to estimation. Low density lipoprotein (LDL) concentration was calculated using Friedewald's formula as previously described (23 (link)). The inter-assay coefficient of variation for commercial controls and normal human pool serum/plasma was 4.9–7.0% for total cholesterol, 6.1–7.7% for triglycerides, and 7.1–12.2% for HDL-c.