Anti cd161 pe cy7

Manufactured by BioLegend

Anti-CD161 PE-Cy7 is a fluorescently-labeled antibody that binds to the CD161 surface antigen. CD161 is a type II transmembrane protein expressed on natural killer cells, a subset of T cells, and other immune cell populations. This antibody can be used to identify and study CD161-expressing cells in flow cytometry applications.

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Lab products found in correlation

Anti cd3 af700, by BioLegend (1 mentions) Ec800, by Sony (1 mentions) Anti cd158d pe, by BioLegend (1 mentions) Anti il 10 pe, by BioLegend (1 mentions) Anti ifn γ fitc, by BioLegend (1 mentions) Horizon brilliant stain buffer, by BD (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti cd3 alexafluor700, by BD (1 mentions)

Spelling variants of this product

Anti‐CD161‐PE (3 citations) CD161-PE (2 citations) Anti-CD161 PE-Cy7 (HP-3G10, (2 citations)

3 protocols using anti cd161 pe cy7

Phenotypic analysis of PBMCs

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Peripheral blood mononuclear cells at 1 × 10⁶ viable cells/mL (final concentration) in complete DMEM/F12 were incubated with anti-CD3 AF700 (Biolegend), anti-CD28 Pacific blue (PB) (Biolegend), anti-CD161 PE-Cy7 (Biolegend), anti-CD158d PE (Biolegend), and anti-TCR Vα24-Jα18 FITC (Biolegend) or the corresponding isotype controls. All antibodies were purchased from Biolegend (United Kingdom). After 20 min at 20°C cells were washed, and fixed with 1% formalin. Data were acquired by flow cytometry (EC800 Sony) and analyzed using FlowJo software.

Maijo M., Ivory K., Clements S.J., Dainty J.R., Jennings A., Gillings R., Fairweather-Tait S., Gulisano M., Santoro A., Franceschi C., Carding S.R, & Nicoletti C. (2018). One-Year Consumption of a Mediterranean-Like Dietary Pattern With Vitamin D3 Supplements Induced Small Scale but Extensive Changes of Immune Cell Phenotype, Co-receptor Expression and Innate Immune Responses in Healthy Elderly Subjects: Results From the United Kingdom Arm of the NU-AGE Trial. Frontiers in Physiology, 9, 997.

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Multiparametric Flow Cytometry of PBMCs

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Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll–Paque PLUS (GE Healthcare, Uppsala, Sweden) density-gradient centrifugation. Cell viability was assessed by trypan blue dye exclusion, and it was always higher than 95%. Cells were resuspended in BD Horizon Brilliant Stain Buffer (BD Life Sciences, San Jose, CA) and stained with the following MoAbs: anti-CD4-BV510 (BD Horizon, San Jose, CA), anti-CD183 (CXCR3)-APC-Cy7 (BioLegend Inc., San Diego CA), anti-CD243 (MDR-1)-PerCp/Cy5.5 (BioLegend), and anti-CD161-PE/Cy7 (BioLegend). Then, cells were fixed, permeabilized with p-formaldehyde 4.0% and saponine 0.1%, and additionally stained with the following MoAbs: anti-IL-10-PE (BioLegend), anti-IFN-γ-FITC (BioLegend), anti-IL-17A-BV421 (BD Horizon), and anti-IL-22-APC (eBioscience Inc., San Diego, CA). Negative controls were designed according to the fluorescence minus-one (FMO) strategy, and cells were analyzed in a FACSCanto flow cytometer (Becton–Dickinson) and with the Flow Jo software (Tree Star Inc, Ashland, OR).

Vitales-Noyola M., Hernández-Castro B., Alvarado-Hernández D., Baranda L., Bernal-Silva S., Abud-Mendoza C., Niño-Moreno P, & González-Amaro R. (2022). Levels of Pathogenic Th17 and Th22 Cells in Patients with Rheumatoid Arthritis. Journal of Immunology Research, 2022, 5398743.

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Staining and Analysis of Human PBMCs

Cited 1 time

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PBMCs were isolated from whole blood of healthy donors (authorized by the Australian Red Blood Cross Service Material Supply Agreement with the University of Melbourne) as described previously (Reantragoon et al., 2013 (link)). Approximately 1.2 × 10⁶ human PBMCs were stained with live/dead discriminator (Live/Dead Fixable Aqua Dead Cell Stain kit; Invitrogen) PE-labeled, human tetrameric, MR1–5-OP-RU, MR1-6-FP or MR1–Ac-6-FP at 5 µg/ml, anti-CD3-Alexa Fluor 700 (BD), and anti-CD161-PE-Cy7 (BioLegend) mAb for 30 min on ice in the dark. Cells were then washed three times with wash buffer (2% fetal calf serum in PBS) and resuspended in fixing solution (2.1% glucose and 1% paraformaldehyde in PBS). Data were acquired using an LSR Fortessa (BD) and analyzed using FlowJo software.

Eckle S.B., Birkinshaw R.W., Kostenko L., Corbett A.J., McWilliam H.E., Reantragoon R., Chen Z., Gherardin N.A., Beddoe T., Liu L., Patel O., Meehan B., Fairlie D.P., Villadangos J.A., Godfrey D.I., Kjer-Nielsen L., McCluskey J, & Rossjohn J. (2014). A molecular basis underpinning the T cell receptor heterogeneity of mucosal-associated invariant T cells. The Journal of Experimental Medicine, 211(8), 1585-1600.

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