11 oh thc

Manufactured by Cerilliant

Sourced in United States

11-OH-THC is a reference standard used for analytical and research purposes. It is a metabolite of tetrahydrocannabinol (THC), the primary psychoactive compound found in cannabis. This reference standard is intended for use in the identification and quantification of 11-OH-THC in various sample matrices through analytical techniques such as chromatography and mass spectrometry.

Automatically generated - may contain errors

Lab products found in correlation

Thccooh, by Cerilliant (3 mentions) Formic acid, by Thermo Fisher Scientific (3 mentions) Cbd d3, by Cerilliant (3 mentions) 11 oh thc d3, by Cerilliant (3 mentions) Thc d3, by Cerilliant (3 mentions) Cbn d3, by Cerilliant (3 mentions) Ammonium acetate, by Merck Group (2 mentions) Thccooh gluc, by Cerilliant (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) δ9 thc, by Cerilliant (2 mentions) Lc ms grade formic acid, by Merck Group (1 mentions) Kinetex c18, by Phenomenex (1 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Merck Group (1 mentions) Thcaa, by Lipomed (1 mentions) Elga purelab ultra analytic purifier, by Siemens (1 mentions) Ammonium acetate, by Thermo Fisher Scientific (1 mentions) 2 propanol, by Thermo Fisher Scientific (1 mentions) Borosilicate glass tubes, by Thermo Fisher Scientific (1 mentions) Thca a, by Cerilliant (1 mentions)

Spelling variants of this product

THC-OH (3 citations) 11-OH-THC-d3 (3 citations) [2H3]-11-OH-THC (2 citations)

6 protocols using 11 oh thc

Analytical Method for Cannabis Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

THC, 11-OH-THC, THCCOOH, THCCOOH-gluc, CBD, CBN, d₃-THC, d₃-11-OH-THC, d₉-THCCOOH, d₃-CBD, d₃-CBN and d₃-THCCOOH-gluc (d₃-THCCOOH-gluc) were purchased from Cerilliant (Round Rock, TX, USA). CBG was obtained from Restek (Bellefonte, PA, USA), THCAA was from Lipomed (Arlesheim, Switzerland), THCVCOOH and THC-gluc from ElSohly Laboratories (Oxford, MS, USA) and THCV from RTI International (Research Triangle Park, NC, USA). Acetonitrile, ammonium acetate and formic acid (LC-MS grade) were acquired from Sigma Aldrich (St. Louise, MO, USA) and methanol (LC-MS grade) from Fischer Scientific (Fair Lawn, NJ, USA). Water was purified in-house by an ELGA Purelab Ultra Analytic purifier (Siemens Water Technologies, Lowell, MA, USA). WAX-S tips (DPX Labs, Columbia, SC, USA) were used to extract samples, and a Kinetex C18 2.1 × 50 mm, 2.6 μm column combined with a 2.1 × 2 mm guard column of identical phase (Phenomenex Inc., Torrance, CA, USA) accomplished analyte separation.

Andersson M., Scheidweiler K.B., Sempio C., Barnes A, & Huestis M.A. (2016). Simultaneous quantification of eleven cannabinoids and metabolites in human urine by liquid chromatography tandem mass spectrometry using WAX-S tips. Analytical and bioanalytical chemistry, 408(23), 6461-6471.

+ Open protocol

+ Expand

Comprehensive Cannabinoid Standard Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stocks of 1 mg/mL of CBD, THC, CBN, 11-OH-THC, CBG, THCV, THCA-A, and THCCOOH-D 3 as well as 100 μg/mL solutions of THCCOOH, THCCOOH-gluc, CBD-D 3 , THC-D 3 , CBN-D 3 , and 11-OH-THC-D 3 were purchased from Cerilliant (Round Rock, TX, USA). A 10-μg/mL THCgluc stock was obtained from ElSohly (Oxford, MS, USA).
Optima LC/MS grade acetonitrile, methanol, 2-propanol, formic acid (FA), and ammonium acetate were obtained from Fisher Scientific (Hampton, NH, USA). Oasis PRiME HLB 96-well solid-phase extraction (SPE) plates with 30 mg sorbent/well, 96-well collection plates, quick-load glass inserts, silicone/PTFE-treated 96-well square plug cap mats, and amber max recovery autosampler vials were acquired from Waters (Milford, MA, USA). Borosilicate glass tubes were from Fisher Scientific and 18.2 MΩ-cm water was dispensed from a Siemens PureLab Ultra purification system.
Blank WB was created by mixing acid citrate dextrose (ACD)packed red blood cells with ABO-compatible plasma and confirming the absence of detectable cannabinoids. Breath matrix consisted of an unused SensAbues ® electret filter material ("pad") cut into 28 cm 2 squares to match the intake surface area of the SensAbues ® collection device.

Hubbard J.A., Smith B.E., Sobolesky P.M., Kim S., Hoffman M.A., Stone J., Huestis M.A., Grelotti D.J., Grant I., Marcotte T.D, & Fitzgerald R.L. (2020). Validation of a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to detect cannabinoids in whole blood and breath. Clinical chemistry and laboratory medicine, 58(5).

+ Open protocol

+ Expand

Quantitative Analysis of Cannabinoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

THC, 11-OH-THC, THCCOOH, CBD, CBN, THC-d₃, 11-OH-THC-d₃, THCCOOH-d₉, CBD-d₃, CBN-d₃, and THCCOOH-glucuronide-d₃ were purchased from Cerilliant (Round Rock, TX, USA). CBG was from Restek (Bellefonte, PA, USA), THCV was from RTI International (Research Triangle Park, NC, USA), and THCVCOOH and THC-glucuronide were acquired from ElSohly Laboratories (Oxford, MS, USA). Ammonium acetate and acetonitrile (LC-MS grade) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methanol and water (LC-MS grade) and formic acid (ACS-grade) were from Fisher Scientific (Fair Lawn, NY, USA). WAX-S tips (1 mL tip containing 20 mg resin and 40 mg salt) were purchased from DPX Labs (Columbia, SC, USA). Chromatography was performed on a Kinetex® C₁₈ column (Phenomenex® Inc., Torrance CA, USA; 2.1 mm × 50 mm, 2.6 μm) combined with a SecurityGuard™ C₁₈ guard column (4 × 2.0 mm).

Scheidweiler K.B., Newmeyer M.N., Barnes A.J, & Huestis M.A. (2016). Quantification of Cannabinoids and their Free and Glucuronide Metabolites in Whole Blood by Disposable Pipette Extraction and Liquid Chromatography Tandem Mass Spectrometry. Journal of chromatography. A, 1453, 34-42.

+ Open protocol

+ Expand

Quantitative Analysis of Cannabinoids

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reference standards (Δ9-THC, 11-OH-THC, CBD, and cannabinol [CBN]) and isotopically labeled internal standards (THC-d3, 11-OH-THC-d3, CBD-d3, CBN-d3) were purchased from Cerilliant (Round Rock, TX). BondElut® certify (Varian, Lake Forest, CA) C-18 cartridges were used for solid-phase extraction. HPLC grade water, 0.1% formic acid in acetonitrile (ACN), and methanol (MeOH) were purchased from Fisher Scientific. Reagent grade ammonium formate and pure sodium hydroxide pellets were purchased from Sigma-Aldrich.
We followed the Wei et al saponification-solid-phase extraction method for sample clean up and extraction with minor modifications.^{15 (link)}Chromatographic separation was achieved on reverse-phase C-18 column (MAC-MOD Ace 5 C-18, 15cm · 2.1mm). The mobile phase consisted of 5 mM ammonium formate in 0.05% formic acid (Solvent A) and ACN in 0.1% formic acid (Solvent B). A gradient elution was performed over 15 minutes at a flow rate of 200 μL/min. Retention times for 11-OH-THC, CBD, THC were 9.35, 10.62, and 11.70 minutes in positive ion mode, respectively. Quantitation of THC, 11-OH-THC, and CBD and isotopically labeled internal standards was achieved on a triple quadrupole mass spectrometer (API-4000, AB Sciex) in positive ion mode. CBN and CBN-d3 were detected in negative ion mode.

Bertrand K.A., Hanan N.J., Honerkamp-Smith G., Best B.M, & Chambers C.D. (2018). Marijuana Use by Breastfeeding Mothers and Cannabinoid Concentrations in Breast Milk. Pediatrics, 142(3), e20181076.

+ Open protocol

+ Expand

Cannabinoid Standards for LC-MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Commercially available standards for delta-8-tetrahydrocannabinol (Δ8THC) (100 μg/mL), Δ9THC (100 μg/mL), 11-OH-THC (100 μg/mL), 11-COOH-THC (100 μg/mL), and delta-9-tetrahydrocannabinol-d3 (Δ9THC-d3) [(100 μg/mL); internal standard (IS)] stock solutions were obtained from Cerilliant (Round Rock, TX, United States). Liquid chromatography-mass spectrometry (LC-MS) grade water, methanol, and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, United States).

Gazarov E.A., Zequeira S., Senetra A.S., Howard J., Sharma A., McCurdy C.R., Lewis J., Bizon J.L, & Setlow B. (2023). Pharmacokinetics of delta-9-tetrahydrocannabinol following acute cannabis smoke exposure in mice; effects of sex, age, and strain. Frontiers in Pharmacology, 14, 1227220.

+ Open protocol

+ Expand

Quantification of Endocannabinoid Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Authentic THC, 11-OH-THC, and 11-COOH-THC standards and their corresponding ²H-containing derivatives were obtained from Cerilliant (Round Rock, TX). THC for animal administration was purchased from Sigma-Aldrich (St. Louis, MO). LC/MS-grade water and methanol were from Honeywell (Muskegon, MI). LC/MS-grade acetonitrile (ACN), isopropanol, and acetone were from Sigma-Aldrich. Formic acid (FA) was from Thermo Fisher (Houston, TX); 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine (18:0 lysophosphatidylcholine, 18:0 LPC) and 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (18:0–20:4 phosphatidylcholine, 18:0–20:4 PC) were from Avanti Polar Lipids (Alabaster, Alabama).

Vozella V., Zibardi C., Ahmed F, & Piomelli D. (2019). Fast and Sensitive Quantification of Δ9-Tetrahydrocannabinol and Its Main Oxidative Metabolites by Liquid Chromatography/Tandem Mass Spectrometry. Cannabis and Cannabinoid Research, 4(2), 110-123.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!