Formalin-fixed and paraffin-embedded tissue sections were stained with anti-CD66b (305102, Biolegend), anti-cleaved caspase-3 (559565, BD Pharmingen, Franklin Lanes, NJ, USA), M30 (12140322001, Roche, Merck, Darmstadt, Germany), anti-IL-8 (MA5-23697, Thermo Fisher Scientific), anti-CD3 (MA5-14524, Thermo Fisher Scientific), anti-CD68 (M087629-2, Dako, Agilent Technologies, Carpinteria, CA, USA) and anti-CD206 (ab64693, Abcam, Cambridge, UK) antibodies for 1 h, followed by nuclear counterstaining with Hematoxylin Gill III (1.05174.0500, Merck). See supplementary material for extended protocol.
Anti il 8
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-IL-8 is a laboratory equipment product used for the detection and quantification of the interleukin-8 (IL-8) protein. It is designed to aid in the analysis of biological samples and support research activities.
Automatically generated - may contain errors
Lab products found in correlation
Ab64693, by Abcam (1 mentions)
Anti cd68, by Agilent Technologies (1 mentions)
Hematoxylin gill 3, by Merck Group (1 mentions)
Anti cd3, by Thermo Fisher Scientific (1 mentions)
Anti cd66b, by BioLegend (1 mentions)
Ma5 14524, by Thermo Fisher Scientific (1 mentions)
M087629 2, by Agilent Technologies (1 mentions)
Anti cleaved caspase 3, by BD (1 mentions)
Anti p53, by Thermo Fisher Scientific (1 mentions)
Anti p erk, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Thermo Fisher Scientific (1 mentions)
Anti α sma, by Thermo Fisher Scientific (1 mentions)
Anti cox 2, by Thermo Fisher Scientific (1 mentions)
Anti bax, by Thermo Fisher Scientific (1 mentions)
Anti bcl 2, by Thermo Fisher Scientific (1 mentions)
Anti p nf κb, by Thermo Fisher Scientific (1 mentions)
Anti nox2, by Thermo Fisher Scientific (1 mentions)
Anti sp1, by Thermo Fisher Scientific (1 mentions)
Anti mmp 2, by Thermo Fisher Scientific (1 mentions)
Anti rac1, by Thermo Fisher Scientific (1 mentions)
4 protocols using anti il 8
1
Immunohistochemical Profiling of Tissue
2
Comprehensive Molecular Signaling Pathway Analysis
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Anti-NOX-2, anti-Rac-1, anti-p47phox, anti-p-53, anti-Bax, anti-Bcl-2, anti-cytochrome c, anti-β-actin, anti-p-I-κBα, anti-p-p38, anti-p-NF-κB, anti-COX-2, anti-IL-8, anti-TGFβ1, anti-p-ERK, anti-Sp1, anti-CTGF, anti-FGF2, anti-uPA, anti-MMP-2, anti-MMP-9, and anti-α-SMA were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Secondary antibodies were obtained from Cell Signaling (Danvers, MA, USA).
3
Immunoblot Analysis of Cell Signaling
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Immunoblots were carried out as previously reported [44 (link)] using polyclonal rabbit antibodies, anti-human anti-p38MAPK, anti-phospho-p38MAPK (Cell Signalling Technology Inc., Danvers, MA, USA), anti-IL8 (ThermoFisher Scientific, Rockford, USA), anti GAPDH (Santa Cruz Biotechnology, Inc, Texas, USA) and mouse antibody anti-VEGF (Santa Cruz Biotechnology).
4
Immunohistochemical Profiling of Gastric Cancer
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According to standard IHC procedures and evaluation methods [16] , tissues fixed in formalin and embedded in paraffin were incubated with polyclonal antibodies against human RABL6 (Abcam, USA, 1:100 dilution), anti-IL-6 (Abcam, USA, 1:200 dilution), anti-IL-8 (Thermofisher, USA, 1:200 dilution), anti-CCL14 (Thermofisher, USA, 1:100 dilution), anti-CCL22 (Thermofisher, USA, 1:100 dilution), anti-CXCL3 (Thermofisher, USA, 1:100 dilution) at 4 ℃ overnight, 1 3 followed by secondary antibodies at 37 ℃, the intensity of immunostaining was evaluated according to the previously reported method [16] .
Tissues fixed were incubated with polyclonal antibodies against human CD68 (Thermofisher, USA, 1:200 dilution), anti-CD20 (Thermofisher, USA, 1:200 dilution), anti-CD8 (Thermofisher, USA, 1:200 dilution), anti-CD177 (Thermofisher, USA, 1:100 dilution), anti-S100 (Thermofisher, USA, 1:100 dilution), the density of infiltrating lymphocytes in gastric cancer tissues and adjacent tissues was evaluated, the tumor nest and corresponding regions around the tumor were measured at 400 × magnification, and then the number of nucleated lymphocytes in each region was counted manually and the average value was calculated [17] .
Tissues fixed were incubated with polyclonal antibodies against human CD68 (Thermofisher, USA, 1:200 dilution), anti-CD20 (Thermofisher, USA, 1:200 dilution), anti-CD8 (Thermofisher, USA, 1:200 dilution), anti-CD177 (Thermofisher, USA, 1:100 dilution), anti-S100 (Thermofisher, USA, 1:100 dilution), the density of infiltrating lymphocytes in gastric cancer tissues and adjacent tissues was evaluated, the tumor nest and corresponding regions around the tumor were measured at 400 × magnification, and then the number of nucleated lymphocytes in each region was counted manually and the average value was calculated [17] .
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