Genomic DNA was extracted from human liver tissues and HCC cell lines (Hep3B, SNU475 and SNU449 cells) using QIAGEN DNA miniprep kits (Valencia, CA, USA) according to the manufacturer's instructions. Then DNA was modified using EZ DNA methylation-direct kits (Zymo Research, Irvine, CA, USA). The HM450 BeadChip array (Illumina, San Diego, CA, USA) analysis was conducted according to the manufacturer's instructions. Methylation levels were reported as β-values, with a range from 0 to 1. All HM450 arrays were processed by LAS Inc. (Daejeon, Korea).
Hm450 beadchip array
Manufactured by Illumina
Sourced in United States
The HM450 BeadChip array is a laboratory equipment product developed by Illumina. It is designed to analyze DNA methylation patterns across the human genome. The core function of this product is to enable researchers to measure the methylation status of over 450,000 CpG sites in a single experiment.
Automatically generated - may contain errors
2 protocols using hm450 beadchip array
1
DNA Methylation Analysis of Liver Tissues and Cell Lines
2
DNA Methylation Analysis of TNBC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DNA was collected from untreated and DAC-treated MDA-MB-231 and MDA-MB-468 cells using the PureLink DNA kit (Invitrogen). Methylation analyses using the HM450 bead chip array (Illumina) was performed by The Centre for Applied Genomics including bisulfite conversion, hybridization, background subtraction, and normalization (Geo Accession GSE103425). β-values for Illumina probes near each gene of interest were extracted from the data, and location determined relative to the transcription start site (TSS).
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