Three groups of MCF-7 cells (4,000 cells/well) in the logarithmic growth phase were inoculated into 96-well plates. After the cells adhered to the wall, various chemotherapeutic drugs were added. The final concentrations of docetaxel (Beijing Solarbio Science & Technology Co., Ltd.) were 0.2,0.4, 0.8, 1.0 and 1.2 µmol/l and the final concentrations of cis-platinum (Beijing Solarbio Science & Technology Co., Ltd.) complexes were 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 µmol/l. Three replicate wells were set at each concentration. Cells in the B.C group were used as control wells. Blank wells without any cells were filled with equal volume (100 µl) of DMEM (cat. no. 01-052-1ACS; Biological Industries). After 24 h of drug treatment, Cell Counting Kit (CCK)-8 (cat. no. ab228554, Abcam) reagent was added at 100 µl per well and mixed, followed by incubation for 1 h at room temperature according to the manufacturer's protocols. Finally, the optical density (OD) at 450 nm was determined for each well using an enzyme-linked immunosorbent assay detection system (HBS-1096c; Nanjing Detielab Experimental Equipment Co., Ltd.). Cell viability and the half maximal inhibitory concentration (IC50) of the two drugs in different groups were calculated using GraphPad Prism version 6 (GraphPad Software).
Cis platinum
Manufactured by Solarbio
Sourced in China
Cis-platinum is a chemical compound used in laboratory applications. It is a platinum-based complex with the formula Pt(NH3)2Cl2. Cis-platinum is often used in research and analytical procedures, but its specific function and intended use should not be extrapolated or interpreted beyond this factual description.
Automatically generated - may contain errors
2 protocols using cis platinum
1
Comparative Cytotoxicity of Docetaxel and Cisplatin in MCF-7 Cells
2
Melanoma Cell Response to RA and Cis
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The human melanoma cell-line A375 was purchased from Cell Bank of Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM medium (Thermo Fisher Scientific, USA) supplemented with 10% FBS (Gibco, USA) and 1% penicillin-streptomycin (Beyotime Institute of Biotechnology, China) in a 5% CO2 incubator at 37°C.
For RA treatment (purity>98%, Sigma, USA), cells that attached to the plate bottom were exposed to different concentrations of RA (0, 50, 100, and 200 μg/ml) for 24, 48 or 72 h according to a previous study [15 ]. For cis-platinum (Cis, purity≥98.5%, Solarbio, China) and RA co-treatment, cells were pre-treated to RA for 24 h, followed by 8 μM Cis co-treatment for another 24 h. For RA and recombinant TACE prodomain (TPD; ADAM17 inhibitor) co-treatment, cells were co-treated with 200 μg/ml RA and 1 μM E01-GS-TPD for 48 h.
For RA treatment (purity>98%, Sigma, USA), cells that attached to the plate bottom were exposed to different concentrations of RA (0, 50, 100, and 200 μg/ml) for 24, 48 or 72 h according to a previous study [15 ]. For cis-platinum (Cis, purity≥98.5%, Solarbio, China) and RA co-treatment, cells were pre-treated to RA for 24 h, followed by 8 μM Cis co-treatment for another 24 h. For RA and recombinant TACE prodomain (TPD; ADAM17 inhibitor) co-treatment, cells were co-treated with 200 μg/ml RA and 1 μM E01-GS-TPD for 48 h.
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