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Ndei and xhoi endonucleases

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NdeI and XhoI are type II restriction endonucleases that recognize and cleave specific DNA sequences. NdeI recognizes and cleaves the palindromic DNA sequence 5'-CA↓TATG-3', while XhoI recognizes and cleaves the palindromic DNA sequence 5'-C↓TCGAG-3'.

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Lab products found in correlation

E coli trans5α, by Transgene (1 mentions) Ni nta agarose resin, by Qiagen (1 mentions) T4 dna ligase, by Transgene (1 mentions) Bl21 de3 competent cells, by Transgene (1 mentions)

Spelling variants of this product

XhoI (381 citations) NdeI and XhoI (5 citations)

2 protocols using ndei and xhoi endonucleases

1

Protein Expression and Purification

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Ni-NTA agarose resin was purchased from Qiagen (Hilden, Germany). Trans5K DNA marker, T4 DNA ligase, E. coli Trans5α and BL21(DE3) competent cells were products of TransGen Biotech (Beijing, China). NdeI and XhoI endonucleases were purchased from New England Biolabs (Ipswich, MA, USA). The substrates of pNPA, pNPB, pNPC, pNPL and pNPP were obtained from Aladdin Biotech (Shanghai, China).
Wang X., Li Z.M., Li Q., Shi M., Bao L., Xu D, & Li Z. (2019). Purification and biochemical characterization of FrsA protein from Vibrio vulnificus as an esterase. PLoS ONE, 14(4), e0215084.
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2

Cloning of Photosynthetic Sensor Kinases

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Coding sequences of full-length Synechocystis sp. PCC6803 Hik2 (slr1147), A. thaliana CSK (At1g67840), and P. tricornutum CSK (PHATRDRAFT_41268) were amplified by using the primer pairs listed in Supplementary Table 3. PCR products of Hik2 and Arabidopsis CSK were digested with NdeI and XhoI endonucleases (New England BioLabs) and cloned into pET-21b (Novagen) expression vector digested with the same enzymes. The Phaeodactylum CSK PCR product was digested with KpnI and XhoI and cloned into pETG-41A (EMBL) expression vector. The identities of the recombinant clones were confirmed by sequencing.
Ibrahim I.M., Wu H., Ezhov R., Kayanja G.E., Zakharov S.D., Du Y., Tao W.A., Pushkar Y., Cramer W.A, & Puthiyaveetil S. (2020). An evolutionarily conserved iron-sulfur cluster underlies redox sensory function of the Chloroplast Sensor Kinase. Communications Biology, 3, 13.
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