Molecular analyst fingerprinting software

In order to determine their clonal relationship, 13 isolates of S. Enteritidis from stool samples and one from water were analysed by pulsed-field gel electrophoresis (PFGE). These 13 stool isolates were chosen for PFGE analysis as representative samples, according to epidemiological interviews. The plugs were prepared according to the protocol described by Ribot et al. [16 (link)] and afterwards restricted with XbaI for 3 h at 37 °C. Salmonella Braenderup H9812 strain was used as a size standard. Electrophoresis was performed using 1.4% certified PFGE agarose gel (Bio-Rad, Germany) in CHEF DRII/III (Bio-Rad, Germany), according to the conditions described in the PulseNet PFGE protocol for Salmonella issued by the US Centers for Disease Control and Prevention. Obtained fingerprinting patterns were analysed by Molecular Analyst Fingerprinting software (Bio-Rad, Germany). Dice similarity coefficients were calculated using pairwise comparison, with optimization and a position tolerance of 1.5%. The dendrogram was created using the unweighted pair group method with arithmetic averages.