E-40 (Sigma-Aldrich Co., St Louis, MO, USA), CCTG (Via Farma, São Paulo, SP, Brazil), PEG-40 (Pharma Special, Itapevi, São Paulo, Brazil), P-407 (Pluronic® F127, Sigma-Aldrich Co.), and CTAB (Sigma-Aldrich Co.) were used to develop the NLCs. Middlebrook 7H9 broth supplemented with oleic acid, albumin, dextrose, and catalase (OADC) (Becton-Dickinson, Franklin Lakes, NJ, USA), resazurin, and rifampicin (Sigma-Aldrich Co.) were used to investigate the in vitro biological activity. Ultrapure water (EMD Millipore Billerica, MA, USA) was used in this study.
P 407 pluronic f127
Manufactured by Merck Group
Sourced in Brazil, United Kingdom
Pluronic® F127 (P-407) is a nonionic, triblock copolymer composed of polyethylene oxide and polypropylene oxide. It is a white, water-soluble, waxy solid that exhibits thermoreversible gelation properties.
Automatically generated - may contain errors
2 protocols using p 407 pluronic f127
1
Nanostructured Lipid Carriers for Anti-TB Therapy
2
P-407 Hyperlipidemia Induction Protocol
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P‐407 (Pluronic F‐127, Cat. no. P2443; Sigma‐Aldrich, Dorset, UK) was dissolved overnight in cooled sterile phosphate‐buffered saline (PBS). Mice were injected intraperitoneally (i.p.) with 200 μl of P‐407 solution (10, 5 or 2·5 mg equivalent to 0·5, 0·25 and 0·125 g/kg, respectively) or PBS every other day. According to the experimental design blood samples were collected into heparin tubes at different time‐points. In P‐407‐treated animals, blood collection was performed 24 h following drug administration. CHOL and TG levels were measured by colorimetric assay using the cholesterol and triglyceride infinity reagent (TR13421 and TR22421, respectively; Thermo‐Scientific, Middletown, VA, USA).
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