For animal studies, 20 male BALB/c nude mice were obtained from the National Laboratory Animal Center (Taipei, Taiwan) and kept in a conventional animal facility. All animal experiments were approved and conducted in accordance with the guidelines of the National Tsing Hua University Institutional Animal Care and Use Committee. Upon arrival, the animals were divided into two groups, the A549luc + Levo group and the A549luc control group. The luciferase expressing A549 cell line (A549luc) was purchased from Caliper Lifesciences Corp. After conventional expansion, A549luc cells (1.5 × 106) were intravenously injected through the tail vein. A luciferin solution (2 mL, 150 mg/mL) (Firefly Luciferin, Caliper Lifesciences Corp, USA) was then injected intraperitoneally. Dissemination of fluorescent cells was probed using an in vivo imaging system (IVIS) (Caliper Lifesciences Corp, USA) every seven days for a period of three months. At each time point, animals were anesthetized using 2% isoflurane and imaged using a cooled CCD camera. Bioluminescent signals (photons/s) of xenografted mice were quantified using the Living Image 3.0 software (Caliper Lifesciences Corp, USA). After three months of monitoring, the animals were euthanized and pathological examinations were carried out.
A549luc
Manufactured by PerkinElmer
Sourced in United States
The A549luc is a laboratory cell line derived from human lung carcinoma cells that have been genetically modified to express luciferase, a bioluminescent reporter protein. The core function of this product is to provide a standardized cell line for research applications involving bioluminescent imaging and analysis.
Automatically generated - may contain errors
4 protocols using a549luc
1
Bioluminescent Xenograft Mouse Model
2
Luciferase-Expressing A549 Cell Line
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Human A549 lung adenocarcinoma cell line stably transfected with luciferase (A549luc) was purchased from Caliper Life sciences Corp. Cells were cultured in DMEM media supplemented with 10 % fetal calf serum (FCS), 100 U/ml penicillin and 100 U/ml streptomycin.
3
Culturing Melanoma and Lung Cancer Cells
4
Primary Tumor Cell Isolation Protocol
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A549-luc, BXPC-3-luc, HT-29-luc, Hela-luc, MDA-MB231-luc, NCIH460-luc, PC-3M-luc, and LL/2-luc-M38 cell lines were all purchased from Caliper Life Sciences. U87MG-luc cells were generated by our group previously [35 ]. The primary culture was derived from freshly isolated tumor cell from patients of two primary lung cancer or two primary breast cancer individually, in Beijing Shijitan Hospital of Capital Medical University. The consent was obtained from the patients before sample collection. The study complied with the Declaration of Helsinki and was approved by the Biomedical Research Ethics Committee of Beijing Shijitan Hospital of Capital Medical University. Briefly, tumors were placed into RPMI1640 medium with antibiotics and anti-fungal immediately following resection. Specimens were washed with cold PBS repeatedly to remove blood clots and tissue debris, and then minced into 0.5- to 1.0-mm pieces. The tissue pieces were digested with collagenase IV, Dnase I and hyaluronidase. Then cells were washed and passed through a 70 μm cell strainer to obtain single-cell population. All agents for cell culture were from Gibco Company (Gaithersburg, MD, USA). Beige-SCID Mice (8 to 10 weeks) were purchased from Vital River Laboratories (VRL, Beijing, China).
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