Whole-cell extracts were prepared with lysis buffer (50 mM Tris-pH7.5, 150 mM NaCl, 1% NP-40, and 5 mM EDTA). Equal amounts of total proteins were separated by SDS-PAGE and transferred to PVDF membranes. Membranes were immunoblotted with anti-FLAG, anti-HA, anti-Myc (Cell Signaling Technology, Danvers, MA, USA), anti-GFP, anti-Gal4, anti-Lamin B1 (Santa Cruz Biotechnology, Dallas, TX, USA), anti-α-tubulin (AbFrontier, Seoul, Republic of Korea), and anti-ERRγ (R&D Systems, Minneapolis, MN, USA) antibodies. Densitometry was performed using ImageJ software (NIH, Bethesda, AR, USA).
Anti errγ
Manufactured by R&D Systems
Sourced in United States
Anti-ERRγ is a laboratory reagent that specifically binds and detects the Estrogen-Related Receptor gamma (ERRγ) protein. It can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of ERRγ in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti ha, by Cell Signaling Technology (1 mentions)
Anti α tubulin, by AbFrontier (1 mentions)
Anti myc, by Cell Signaling Technology (1 mentions)
Anti gal4, by Santa Cruz Biotechnology (1 mentions)
Anti lamin b1, by Santa Cruz Biotechnology (1 mentions)
Anti flag, by Cell Signaling Technology (1 mentions)
Anti gfp, by Santa Cruz Biotechnology (1 mentions)
Chromatin ip kit, by Cell Signaling Technology (1 mentions)
2 protocols using anti errγ
1
Immunoblot Analysis of Protein Abundance
2
Chromatin Immunoprecipitation of ERRγ
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChIP was performed using the Chromatin IP kit (#9004, Cell Signaling Technology, Danvers, MA, USA). Soluble chromatin was subjected to immunoprecipitation using anti-IgG or anti-ERRγ (#PP-H6812-00, R&D systems, Minneapolis, MN, USA). After DNA recovery, DNA was analyzed by PCR using primers against the hepcidin promoter (forward: 5′-GAGCCACAGTGTGACATCAC-3′; reverse: 5′-GTCTAGGAGCCAGTCCCAGT-3′).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!