Uvmini 1280 spectrophotometer

Fibrin polymerization of recombinant γT277R and γY278H fibrinogens catalyzed by human α-thrombin (Enzyme Research Laboratories, South Bend, MA, USA) was performed as described previously [46 (link)]. Briefly, fibrinogen (90 µL at 0.20 mg/mL) in 20 mM HBS buffer was mixed with thrombin (10 µL at 0.5 U/mL) at room temperature. The turbidity change at 350 nm was followed using a UVmini-1280 spectrophotometer (Shimadzu, Tokyo, Japan), and reactions were performed in triplicate for each sample. For reaction parameters, the lag time and maximum slope were obtained from turbidity curves. Wild-type and γR275C fibrinogens were used to compare these functions.

The UV–Vis absorption spectra of the NPs and the reaction products were determined using a UVmini-1280 spectrophotometer (SHIMADZU, Kyoto, Japan). Transmission electron microscopy (TEM) images of the AgNPs were obtained using a HT7700 TEM (HITACHI, Tokyo, Japan). The Raman spectra were obtained using a portable Raman spectrometer (BWS465-785S, B&W TEK, Newark, DE, USA) equipped with a 785 nm laser. The laser power was 150 mW, and the exposure time for data collection was 5 s.