Jsm 7001f sem system

A JSM-7001F SEM system (JEOL, Tokyo, Japan), equipped with a Schottky-type field emission electron gun was used in the DET experiment. To obtain the EBSP signal, we used an electron beam of 30 keV accelerating voltage, an 87 pA beam current, and a 17 mm working distance at a 70-degree angle relative to the EC sample stage. We detected EBSDs by the EBSD detector on the JSM-7001F system, which was custom designed by TSL Solutions (DVC1412-FW-T1-EX, TSL Solutions; Sagamihara, Japan). The detector included an image intensifier (V8070U-74, Hamamatsu Photonics; Hamamatsu, Japan). Each spot was irradiated with the electron beam for 1.5 s. The EBSP was obtained on the phosphor screen of the detector, intensified, and recorded with a CCD camera using a 60 ms shutter speed. The Euler angles, which indicate the rotational relationship between the sample stage coordinate system and the crystal lattice coordinate system of the gold nanorod, were obtained from the EBSP using OIM Analysis (EDAX Inc.; Mahwah, NJ, USA). From the Euler angles of adjacent EBSP frames, the single-axis rotation angle ω and the angles α, β, and γ of the principal lattice vectors a, b, and c, were calculated as described previously [15 (link)].

The surface characteristics of dried blood spheroids under different drying conditions were studied with a SEM. Dried whole blood spheroids deposited on paper substrates were coated with a thin layer of gold (10 nm). SEM images were acquired using a JEOL JSM 7001F SEM system under vacuum with an accelerating voltage of 10 kV.

The copolymer mixed gel solution was prepared at 5 wt % in P–Lys–Ala–PLX and 1 wt % in Pluronic F-127 that allowed full equilibration at 4 °C overnight for future use. The hydrogel microstructure was examined using a JEOL JSM-7001F SEM system (Peabody, MA, USA). Briefly, hydrogels were gelled at 37 °C and then submerged in liquid nitrogen prior to lyophilization. To investigate the cross section, lyophilized hydrogels were carefully cut open using a scalpel blade.