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Phosphate buffer saline (pbs)

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Phosphate buffer saline (PBS) is a commonly used buffer solution in biological research. It is a balanced salt solution that maintains a physiological pH and osmolarity, providing a suitable environment for various biological applications. PBS is used to dilute, wash, or store biological samples, cells, or proteins, helping to preserve their structural and functional integrity.

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Lab products found in correlation

Trypsin edta, by Harvard Bioscience (2 mentions) Thiazolyl blue tetrazolium bromide mtt, by Merck Group (2 mentions) Ellagic acid, by Merck Group (1 mentions) Eugenol, by Merck Group (1 mentions) Piperine, by Merck Group (1 mentions) Propyl gallate, by Merck Group (1 mentions) Phosphoric acid, by RCI Labscan (1 mentions) Gallic acid, by Merck Group (1 mentions) Myristicin, by Merck Group (1 mentions) Brazilin, by Merck Group (1 mentions) Nitroblue tetrazolium nbt, by Merck Group (1 mentions) Raw264, by American Type Culture Collection (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Butylated hydroxytoluene bht, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trypan blue, by Thermo Fisher Scientific (1 mentions) Clarithromycin, by Merck Group (1 mentions) Roswell park memorial institute rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin p s, by Harvard Bioscience (1 mentions) Katoiii, by RIKEN BioResource Center (1 mentions)

Close spelling variants of this product

Phosphate saline buffer (PBS, (3 citations) PBS buffer (3 citations)

5 protocols using phosphate buffer saline (pbs)

1

Antioxidant and Anti-inflammatory Assays

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Brazilin, ellagic acid, eugenol, myristicin, piperine, gallic acid, and propyl gallate (purity > 98%) were purchased from Sigma-Aldrich (MO, USA). Folin-Ciocalteus’s phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and butylated hydroxytoluene (BHT) were purchased from Fluka (Munich, Germany). Dimethyl sulfoxide (DMSO), acetonitrile, and phosphoric acid were purchased from RCI Lab Scan (Bangkok, Thailand). Purified water was prepared by Milli Q®system from Millipore (Bedford, MA, USA).
Murine macrophage leukemia cell line (RAW 264.7: ATCC® TIB-71™) and Human promyelocytic leukemia cells (HL-60) were purchased from American Type Culture Collection (VA, USA). Roswell Park Memorial Institute medium 1640 (RPMI-1640), fetal bovine serum (FBS), penicillin-streptomycin, phosphate buffer saline (PBS), and trypsin-EDTA were purchased from Biochrom (MA, Germany). Lipopolysaccharide (LPS), 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium bromide (MTT) and nitroblue tetrazolium (NBT) were purchased from Sigma-Aldrich. (MO, USA).
Panchakul C., Thongdeeying P., Itharat A., Pipatrattanaseree W., Kongkwamcharoen C, & Davies N.M. (2023). Analytical determination, antioxidant and anti-inflammatory activities of Bhamrung-Lohit a traditional Thai medicine. Research in Pharmaceutical Sciences, 18(4), 449-467.
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2

Standardizing H. pylori Infection Assays

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H. pylori strain (ATCC 43504) and six clinical isolates of H. pyloriwere obtained from human stomach biopsy specimens kindly provided by Dr. Ratha-korn Vilaichone, Faculty of Medicine, Thammasat University, Thailand. Mouse macrophage leukemia-like cell line (RAW 264.7 and ATCC TIB-71™; Catalog No. 302002.) was obtain from Assoc. Prof. Dr. Arunporn Itharat, Faculty of Medicine, Thammasat University, Thailand. Human gastric carcinoma cell lines (Kato III); catalog no. RCB2088) were purchased from RIKEN BRC, Japan. Columbia agar supplemented with 5% sheep's blood was purchased from RPD, Thailand. Brain heart infusion (BHI) and anaeropack were purchased from Difco, USA, and Mitsubishi gas chemical America, USA, respectively. Clarithromycin, dimethylsulfoxide (DMSO), lipopolysaccharide from E. coli O55 : B5 (LPS), sulphorhodamine B (SRB), thiazolyl blue tetrazolium bromide (MTT), and butylated hydroxytoluene (BHT) were purchased from Sigma-Aldrich, MO, USA. Roswell Park Memorial Institute (RPMI) 1640, fetal bovine serum (FBS), trypsin-EDTA, and trypan blue were purchased from Gibco BRL, NY, USA. Penicillin-streptomycin (P/S) and phosphate buffer saline (PBS) were purchased from Biochrom, MA, Germany. Analytical grade reagents (e.g., hydrochloric acid and isopropanol) were purchased from Labscan Limited, Bangkok, Thailand.
Suthisamphat N., Dechayont B., Phuaklee P., Prajuabjinda O., Vilaichone R.K., Itharat A., Mokmued K, & Prommee N. (2020). Anti-Helicobacter pylori, Anti-Inflammatory, Cytotoxic, and Antioxidant Activities of Mace Extracts from Myristica fragrans. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 7576818.
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3

Functionalization of Hyperbranched Polyester

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Hyperbranched aliphatic polyester BoltornTM H40, BH40 (Mn = 5100 g mol−1, Mw/Mn = 1.8, hydroxyl number = 485 mg KOH/g), bearing 44 hydroxyl end groups was kindly donated by Perstorp AB, Sweden. To remove low molecular weight fractions, BH40 was further fractionated by precipitation, following the procedure described in the literature [35 (link)]. High-purity solvents, such as pyridine, acetone, diethyl ether and dimethylformamide (DMF), were purchased from Merck KGaA (Calbiochem®, Darmstadt, Germany). Succinic anhydride, sodium bicarbonate (NaHCO3), lithium bromide (LiBr), dialysis tubes (M.W. cut-off: 1200), vancomycin hydrochloride (VC), minocycline hydrochloride (MC) and tetracycline base (TC) were purchased from Sigma-Aldrich Ltd. (Poole, UK), while phosphate buffer saline (PBS) was obtained from Biochrom (Berlin, Germany).
Sideratou Z., Biagiotti M., Tsiourvas D., Panagiotaki K.N., Zucca M.V., Freddi G., Lovati A.B, & Bottagisio M. (2022). Antibiotic-Loaded Hyperbranched Polyester Embedded into Peptide-Enriched Silk Fibroin for the Treatment of Orthopedic or Dental Infections. Nanomaterials, 12(18), 3182.
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4

Nanocarrier-mediated Doxorubicin Delivery

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Multi-walled carbon nanotubes containing carboxyl groups > 8% w/w (CNTs), hyperbranched polyethyleneimine (PEI) of 5000 Da (Lupasol® G100, water-free, 99%) and 25,000 Da molecular weight (Lupasol® WF, water-free, 99%) and doxorubicin hydrochloride were kindly donated by Glonatech S.A (Athens, Greece), BASF (Ludwigshafen, Germany) and Regulon S.A. (Athens, Greece), respectively. 1H-Pyrazole-1-carboxamidine hydrochloride, N,N-diisopropylethylamine (DIPEA), thiazolyl blue tetrazolium bromide (MTT), rhodamine B isothiocyanate and dialysis tubes (M.W. cut-off: 1200) were purchased from Sigma-Aldrich Ltd. (Poole, UK). D-MEM low glucose with phenol red, fetal bovine serum (FBS), penicillin/streptomycin, L-glutamine, phosphate buffer saline (PBS) and trypsin/EDTA were purchased from Biochrom (Berlin, Germany). FITC Annexin V Apoptosis Detection Kit with 7-AAD was purchased from BioLegend Way (San Diego, CA, USA). High-purity solvents such as N,N-dimethylformamide (DMF), diethyl ether, methanol and isopropanol were obtained from Merck KGaA (Calbiochem®, Darmstadt, Germany).
Lyra K.M., Kaminari A., Panagiotaki K.N., Spyrou K., Papageorgiou S., Sakellis E., Katsaros F.K, & Sideratou Z. (2021). Multi-Walled Carbon Nanotubes Decorated with Guanidinylated Dendritic Molecular Transporters: An Efficient Platform for the Selective Anticancer Activity of Doxorubicin. Pharmaceutics, 13(6), 858.
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5

Reconstitution of 3D Collagen I Matrices

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The reconstitution of 3D Coll I matrices was performed on PSMA-coated 13 mm glass coverslips. Coll I matrices at the concentration of 1.5, 2.0 and 2.5 mg/ml were reconstituted by mixing rat tail Coll I solution (Corning, Berlin, Germany) with phosphate buffer with an ionic strength of 250 mM at pH 7.5. Coll I solution was prepared on ice to inhibit early fibrillation. Subsequently, 430 µl Coll I solution was transferred onto PSMA-coated coverslips and 400 µl were subsequently removed (30 µl of Coll I solution remained on the coverslip). Coll I fibrillation was carried out at 37 °C, 5% CO2 and 95% humidity for 90 min. To prevent the matrix from drying, matrices were kept in phosphate buffer saline (PBS; Biochrom, Berlin, Germany) prior to use.
Sapudom J., Ullm F., Martin S., Kalbitzer L., Naab J., Möller S., Schnabelrauch M., Anderegg U., Schmidt S, & Pompe T. (2017). Molecular weight specific impact of soluble and immobilized hyaluronan on CD44 expressing melanoma cells in 3D collagen matrices. Acta biomaterialia, 50.
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