VFA levels were tested by Headspace Sampler Gas Chromatography (Agilent Technologies, United States) using the method of Thanh et al. (2009) (link). The commercial standards of acetic acid, butyrate, propionic acid, isovalerate, isobutyric acid, and valerate treated as external standards, were purchased from China Sinopharm Chemical Reagents Co., Ltd. (Beijing, China). Metaphosphoric acid were acquired from Shanghai Aladdin Biotech Inc. (Shanghai, China). A mixture of 1 g colon content with 6% phosphorous acid (m/v, 1:3) was injected into an Agilent Technologies GC7890 Network System (Agilent Technologies, United States) equipped with a 30 m × 0.25 mm × 0.25 μm column (HP-FFAP, Agilent Technologies) for flame ionization (Yang et al., 2018 (link)).
Headspace sampler gas chromatography
Manufactured by Agilent Technologies
Sourced in United States
The Headspace Sampler Gas Chromatography is a laboratory instrument designed to efficiently transfer volatile compounds from a sample into the gas chromatography system for analysis. It automates the sample introduction process, allowing for consistent and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Gc7890 network system, by Agilent Technologies (2 mentions)
7890a network system, by Agilent Technologies (2 mentions)
Db 624, by Agilent Technologies (2 mentions)
Hp ffap, by Agilent Technologies (1 mentions)
6890n network system, by Agilent Technologies (1 mentions)
Isobutyric acid, by Maclin Biochemical Technology (1 mentions)
Metaphosphoric acid, by Maclin Biochemical Technology (1 mentions)
Acetic acid, by Maclin Biochemical Technology (1 mentions)
Propionic acid, by Maclin Biochemical Technology (1 mentions)
Butyric acid, by Maclin Biochemical Technology (1 mentions)
Isovaleric acid, by Maclin Biochemical Technology (1 mentions)
5 protocols using headspace sampler gas chromatography
1
Analyzing Volatile Fatty Acids in Colon Contents
2
Quantifying Gut Short-Chain Fatty Acids
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As in our previous study [2 (link)], the colonic and fecal concentrations of SCFAs (acetate, propionate, isobutyrate, butyrate, isovalerate, and valerate) were measured using Headspace Sampler Gas Chromatography (Agilent Technologies, Santa Clara, CA, USA). A 1 g sample was diluted with 4 mL of sterile water and centrifuged at 10,000× g and 4 °C for 15 min. The obtained supernatant was mixed with metaphosphoric acid (1:4, m/v). Finally, the supernatant was injected into the Agilent Technologies 6890N Network System (Agilent Technologies).
3
Cecal VFA Quantification by GC
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Following the assay method of Yang et al. (2019) (link), the concentration of cecal VFA was estimated by Headspace Sampler Gas Chromatography (Agilent Technologies) using a method by Thanh et al. (2009) . One gram of cecum content was dissolved in water, and the supernatant was extracted after centrifugation to mix with 25% (m/v, 1:3) phosphorous acid. The concentration of VFA were measured using an Agilent Technologies 7890A Network System (Agilent Technologies) equipped with a 30 m × 0.32 mm × 1.8 μm column (DB-624; Agilent Technologies) and flame ionization detector.
4
Quantifying Cecal Short-Chain Fatty Acids
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The concentration of cecal short-chain fatty acid (SCFA) was estimated via headspace sampler gas chromatography (Agilent Technologies, Wilmington, DE, USA) using the method of Thanh et al. [21 (link)]. The 0.5 g sample was dissolved in 1 mL of water. After centrifugation at 12,500× g for 5 min, the supernatant was extracted and mixed with 25% phosphoric acid. The concentration of SCFAs was determined via an Agilent Technologies 7890A Network System equipped with a 30 m × 0.32 mm × 1.8 μm column (DB-624) and flame ionization detector.
5
Volatile Fatty Acid Quantification
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The concentrations of VFAs were determined using headspace sampler gas chromatography (Agilent Technologies, Wilmington, DE, USA) according to previous studies (Thanh et al., 2009 (link); Yang et al., 2019 (link)). The external standards of VFAs (acetic acid, propionic acid, butyric acid, isobutyric acid, valerate acid, and isovaleric acid) and metaphosphoric acid were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). One gram of cecal content was blended with 6% phosphorous acid (m/v, 1:4) and injected into Agilent Technologies GC7890 Network System, equipped with a 30 m × 0.25 mm × 0.25 μm column with a flame ionization detector.
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