Ft ir 410 infrared spectrometer

SCCA, SCCA capture antibody (Ab₁), and SCCA detection antibody (Ab₂) were purchased from Beijing DINGGUO CHANGSHENG biotechnology CO. LTD (China). Adamantanecarboxylic acid (ADA-COOH) (99%) was obtained from J&K Chemical. Mercapto-β-cyclodextrin (HS-β-CD) was obtained from Shandong Zhiyuan Biotechnology Ltd. (China). Bovine serum albumin (BSA) was obtained from Sigma-Aldrich (Beijing, China). MBI was purchased from J&K Chemical (Beijing, China). Phosphate buffered solutions (PBS, 1/15 mol·L⁻¹ KH₂PO₄ and 1/15 mol·L⁻¹ Na₂HPO₄) were used as electrolyte for all electrochemistry measurements. All other chemical reagents were analytical reagents grade and directly used without further purification.
All electrochemical measurements were achieved on a RST 5200F electrochemical workstation (Zhengzhou Shiruisi Instrument Co. Ltd., China). Transmission electron microscope (TEM) images were recorded by a JEOL-1400 microscope (JEOL, Japan). Scanning electron microscope (SEM) images were obtained from JSM-6700F microscope (JEOL, Japan). FT-IR spectra were collected by using a FT-IR-410 infrared spectrometer (JASCO, Japan). Surface area measurements were performed on Micromeritics ASAP 2020M surface area and porosity analyzer (Quantachrome, United States).

Fe₃O₄ nanoparticles were purchased from Aladdin Chemical Co., Ltd. Zinc acetate (Zn(Ac)₂) was purchased from Tianjin Hongyan Chemical Reagent Factory. Triethanolamine was purchased from Guangcheng Chemical Reagent Co., Ltd. (Tianjin). L-Cys was purchased from Yunxiang Chemical Industry Co., Ltd. Absolute ethyl alcohol was purchased from Fuyu Chemical Reagent Factory. All other reagents used in this study were analytical grade, and ultrapure water was used in the preparation of all solutions.
Transmission electron microscope (TEM) images were obtained from a Tecnai G220 TEM (FEI Company, USA). Energy Dispersive X-Ray Spectroscopy (EDS) was recorded by JEOL JSM-6700 F microscope (Japan). FT-IR spectra were collected using a FT-IR-410 infrared spectrometer (JASCO, Japan). Ultraviolet absorption spectra were obtained from a Lambda35 UV-Vis spectrophotometer (PerkinElmer, America). Fluorescence spectra were obtained from a LS-55 fluorescence spectrophotometer (PerkinElmer, America).