Anti cd80 pe clone 16 10a1

Manufactured by BD

Sourced in United States

Anti-CD80 PE (clone 16-10A1) is a fluorochrome-conjugated antibody that binds to the CD80 cell surface protein. CD80 is a costimulatory molecule expressed on antigen-presenting cells and plays a role in regulating T-cell activation. This product is intended for use in flow cytometry and other immunoassay applications.

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Lab products found in correlation

Facsaria 3 instrument, by BD (1 mentions) Anti cd11c pe cy7 clone hl3, by BD (1 mentions) Anti cd86 af700 clone gl1, by BD (1 mentions) Fc block clone 2.4g2, by BD (1 mentions) Anti cd11c bv421 clone n418, by BioLegend (1 mentions)

Spelling variants of this product

CD80-PE (60 citations) Anti-CD80 (43 citations) Anti-CD80-PE (36 citations) CD80 (16-10A1; (6 citations) Anti-CD80 (16-10A1, (3 citations) Anti‐CD80 (clone 16‐10A1; (2 citations) Anti-CD80-PE (16-10A1, (2 citations) CD80 (clone 16-10A1), (2 citations) Anti-mouse CD80-PE (clone 16-10A1) (2 citations)

2 protocols using anti cd80 pe clone 16 10a1

Characterization of PLGA NP-Treated BMDCs

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Prior to analysis, BMDCs were seeded in RPMI complete medium at a density of 2 × 10⁵ cells per well on a 24-well plate. Cells were treated with PLGA NPs for 24 h, then washed with PBS and collected using trypsin-EDTA solution. Cells were stained for 20 min at 4 °C in Staining Buffer with anti-CD11c PE-Cy7 (clone HL3), anti-MHC class II (I-A/I-E) APC/Cy7 (clone M5/114.15.2), anti-CD80 PE (clone 16-10A1), anti-CD86 AF700 (clone GL1) antibodies, and analyzed using FACS AriaIII instrument (all from BD biosciences, Franklin Lakes, NJ, USA). Minimum 10.000 CD11c+ MHCII+ cells were analyzed for each sample.

Tukhvatulin A., Dzharullaeva A., Erokhova A., Zemskaya A., Balyasin M., Ozharovskaia T., Zubkova O., Shevlyagina N., Zhukhovitsky V., Fedyakina I., Pruss I., Shcheblyakov D., Naroditsky B., Logunov D, & Gintsburg A. (2020). Adjuvantation of an Influenza Hemagglutinin Antigen with TLR4 and NOD2 Agonists Encapsulated in Poly(D,L-Lactide-Co-Glycolide) Nanoparticles Enhances Immunogenicity and Protection against Lethal Influenza Virus Infection in Mice. Vaccines, 8(3), 519.

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Immunophenotyping of Murine Cells

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Cells were blocked with Fc block (clone 2.4G2; BD Biosciences) and stained with anti-H-2K^b biotinylated (clone KH95; BD Biosciences), anti-MHCII-IA/IE A647 (clone M5/114.15.2; BioLegend) or A700 (clone 56-5321-82; eBioscience), anti-CD80 PE (clone 16-10A1; BD Biosciences), and anti-CD11c BV421 (clone N418; BioLegend) or BV711 (clone 563048; BD-Biosciences). Stained cells were acquired on a BD LSR Fortessa cell analyzer and analyzed with FlowJo (Treestar) software.

Maschalidi S., Nunes-Hasler P., Nascimento C.R., Sallent I., Lannoy V., Garfa-Traore M., Cagnard N., Sepulveda F.E., Vargas P., Lennon-Duménil A.M., van Endert P., Capiod T., Demaurex N., Darrasse-Jèze G, & Manoury B. (2017). UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells. Nature Communications, 8, 1640.

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