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Human mini elisa development kits

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The Human Mini ELISA Development kits are laboratory assay kits designed to quantify specific analytes in human samples. These kits provide the basic components required to develop and optimize an ELISA (Enzyme-Linked Immunosorbent Assay) for target analyte measurement.

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ELISA Development Kit (45 citations) ELISAs (35 citations) Mini ELISA Development Kits (6 citations) Human Mini ELISA kits (2 citations)

2 protocols using human mini elisa development kits

1

Cytokine modulation by nanoparticles

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Freshly isolated PBMCs from healthy donors (1 × 106 cells/ml) were incubated with empty NPs; SHP‐1 and Cbls siRNA‐loaded NPs; PBS (untreated); or PHA and LPS as positive controls. Following incubation, the supernatant from each sample was collected. The levels of human cytokines TNF‐α, IL‐6, IL‐10, and IFN‐γ were determined using human Mini ELISA Development kits (PeproTech).
Biber G., Sabag B., Raiff A., Ben‐Shmuel A., Puthenveetil A., Benichou J.I., Jubany T., Levy M., Killner S, & Barda‐Saad M. (2021). Modulation of intrinsic inhibitory checkpoints using nano‐carriers to unleash NK cell activity. EMBO Molecular Medicine, 14(1), e14073.
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2

Cytokine Profiling of PBMC Responses

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Freshly isolated PBMCs from healthy donors (1 × 106 cells/ml) were incubated for 24 h with either vehicle only (DMSO) as control, 40 µM of SMC #13, or left untreated. For positive control, cells were incubated with 7 µg/ml PHA for 96 h After incubation, the supernatant from each sample was collected. The levels of human cytokines, IL-6, IL-10, TNF-α, and IFN-γ were determined using Human Mini ELISA Development kits (PeproTech).
Biber G., Ben-Shmuel A., Noy E., Joseph N., Puthenveetil A., Reiss N., Levy O., Lazar I., Feiglin A., Ofran Y., Kedmi M., Avigdor A., Fried S, & Barda-Saad M. (2021). Targeting the actin nucleation promoting factor WASp provides a therapeutic approach for hematopoietic malignancies. Nature Communications, 12, 5581.
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