Noran x ray detector

The in vivo SEM/EDX analysis for illustration and measurements was conducted as previously described by Jung et al. [43 (link)]. In brief, the element distribution analysis was conducted via a LEO Gemini 1530 with a field-emission gun (Carl Zeiss AG, Jena, Germany). Therefore, the trans-sections were initially precoated using carbon and immediately transferred to scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The EDX maps (256 × 196 pixels) were recorded using a Thermo Noran X-ray detector in combination with the ThermoFisher Scientific software Noran System Six. Therefore, voltage was set to 5 kV for imaging and 10 kV for EDX mapping.

The morphology and element distribution of the transection were characterized by SEM/EDX using a LEO Gemini 1530 with a field-emission gun from Carl Zeiss AG (Jena, Germany). Samples were precoated with carbon prior to scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) to prevent the effect of charging on the samples. The EDX maps (256 × 196 pixels) were acquired with a Thermo Noran X-ray detector and the Thermo Fisher Scientific software Noran System Six. The applied voltage was set to 5 kV for imaging and 10 kV for EDX mapping.