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Anti ha peroxidase antibody

Manufactured by Abcam
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Sourced in United Kingdom

The Anti-HA-peroxidase antibody is a monoclonal antibody that is conjugated with horseradish peroxidase (HRP). It is designed to detect the hemagglutinin (HA) epitope tag, which is commonly used to identify and track recombinant proteins in a variety of experimental systems.

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Lab products found in correlation

Complete protease inhibitor cocktail, by Merck Group (1 mentions) Anti ha agarose, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-HA (122 citations) Anti-HA antibody (58 citations) Anti-HA-peroxidase (2 citations)

2 protocols using anti ha peroxidase antibody

1

Expression Analysis of CaWRKY Fused Proteins

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To test the expression level of fused protein, A. tumefaciens GV3101 cells harbouring CaWRKY40‐HA, CaWRKY27‐HA, or CaWRKY58‐HA were coinfiltrated with pCaWRKY40::GUS into pepper leaves. Infiltrated plants were grown in the greenhouse for another 2 days. The total protein of the infiltrated leaves was extracted using protein extraction buffer (10% glycerol, 25 mM Tris‐HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 2% Triton X‐100, 10 mM DTT, 1 × complete protease inhibitor cocktail [Sigma‐Aldrich], and 2% [wt/vol] polyvinylpolypyrrolidone [Choi et al., 2012]). The proteins were analysed by immunoblotting using an anti‐HA‐peroxidase antibody (Abcam).
Liu Z., Shi L., Yang S., Qiu S., Ma X., Cai J., Guan D., Wang Z, & He S. (2020). A conserved double‐W box in the promoter of CaWRKY40 mediates autoregulation during response to pathogen attack and heat stress in pepper. Molecular Plant Pathology, 22(1), 3-18.
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2

Immunoprecipitation of HA-tagged Proteins

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Total protein extracts were incubated with anti-HA agarose (Thermo Fisher Scientific, Waltham, MA, USA) overnight at 4 °C. Beads were collected and washed with Tris-buffered saline and Tween-20 (0.05%). Eluted proteins were analyzed by immune-blotting using an anti-HA–peroxidase antibody (Abcam, Cambridge, UK).
Ifnan Khan M., Zhang Y., Liu Z., Hu J., Liu C., Yang S., Hussain A., Furqan Ashraf M., Noman A., Shen L., Xia X., Yang F., Guan D, & He S. (2018). CaWRKY40b in Pepper Acts as a Negative Regulator in Response to Ralstonia solanacearum by Directly Modulating Defense Genes Including CaWRKY40. International Journal of Molecular Sciences, 19(5), 1403.
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