Mopc 21 400101

T-cell migration assays were performed using 24-well transwell migration plates with a 6.5 mm insert and 5 μM pores (Corning, 3421). Either 10 ng/mL recombinant human IL-8 (BioTechne 208-IL, Abingdon, UK) or conditioned media from SKOV-3 cells (overnight culture in 0.1% FBS DMEM) was added in 500 μL RPMI to the lower chamber and 5 × 10⁵ T-cells in 200 μL media to the upper chamber. T-cell migration to the bottom chamber was quantified after 2 h by counting the number of migrated cells from triplicate wells. In some experiments, using conditioned media, an anti-hIL-8 blocking antibody (BioTechne MAB208, Abingdon, UK) or mouse IgG1 isotype control (Biolegend clone MOPC-21 400101, London, UK), was included in the bottom chamber at 2.5 μg/mL.