Ocug 1 cell line

The NOZ cell line and OCUG-1 cell line were purchased from the Health Science Research Resources Bank (Osaka, Japan). The monocytic THP-1 cell line were purchased from cell bank of the Chinese Academy of Science (Shanghai, China). HEK293T cell line and HepG2 cell line was purchased from American Type Culture Collection (ATCC). HUVEC cell line was gifted by Prof. Jian Hou (Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China). HUVEC primary cell was gifted by Prof. Lan Zhang (Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China). The NOZ was cultured in William's E (Gibco, USA), OCUG-1 in high glucose DMEM (Gibco, USA), HEK293T and TPH1 in RPMI 1640 (Gibco, USA), HUVEC cell line in DMEM/F12 (Gibco, USA), HUVEC primary cell in ECM (ScienCell, USA). All cells were cultured in medium supplied with 10%(v/v) fetal bovine serum, 100 μg/mL streptomycin and 100 U/mL penicillin (Hyclone) in a humidified incubator (5%CO2, 37 ℃). In addition, the medium which was used to cultured HUVEC primary cell should supplied with ECGS (ScienCell, USA). Fibrinogen was purchased from Sigma-Aldrich. Recombinant Human ICAM-1 protein (RP00272) was purchased from ABclonal.