Proteins were separated in 4%–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis in reducing conditions and transferred on polyvinylidene difluoride membranes (MilliporeSigma, ISEQ00010). After blocking with 5% milk, membranes were probed with rabbit anti–human Fn (Epitomics, 1574-1), mouse anti–human collagen type I (Proteintech, 66761-1), rabbit anti–human TFRC (Zen BioScience, 619871), rabbit anti–human TGase2 (Abcam, ab64771), mouse anti–human TGase2 (Abcam, ab2386), rabbit anti–human RhoA (ABclonal, A13947), mouse anti–human RhoA (Santa Cruz Biotechnology Inc., sc-418), mouse anti–human IgA antibody (Southern Biotech, B3506B4), rabbit anti–human GAPDH (Bioworld Technology, AP0063), rabbit anti–human β-tubulin (ZEN Bio, 380628), rabbit anti–human Na+/K+ ATPase1 (ABclonal, A7878), or rabbit anti–Flotillin 2 (GeneTex, GTX114411) and detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies and ECL-Plus detecting system (GE Healthcare).
Mouse anti human collagen type 1
Manufactured by Proteintech
The mouse anti-human collagen type I is a primary antibody that specifically recognizes and binds to human collagen type I. Collagen type I is a major structural protein found in various connective tissues, including skin, bone, and tendons. This antibody can be used in applications such as immunohistochemistry, Western blotting, and ELISA to detect and quantify the presence of collagen type I in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab64771, by Abcam (2 mentions)
Ab2386, by ABclonal (2 mentions)
Rabbit anti human gapdh, by Bioworld Technology (2 mentions)
Rabbit anti human tfrc, by ZenBio (2 mentions)
Mouse anti human rhoa, by Santa Cruz Biotechnology (2 mentions)
Mouse anti human iga antibody, by Southern Biotech (2 mentions)
Rabbit anti human β tubulin, by ZenBio (2 mentions)
Ecl plus detecting system, by GE Healthcare (2 mentions)
Iseq00010, by Merck Group (2 mentions)
2 protocols using mouse anti human collagen type 1
1
Protein Expression Analysis by Western Blot
2
Protein Expression Analysis by Western Blot
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Proteins were separated in 4%–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis in reducing conditions and transferred on polyvinylidene difluoride membranes (MilliporeSigma, ISEQ00010). After blocking with 5% milk, membranes were probed with rabbit anti–human Fn (Epitomics, 1574-1), mouse anti–human collagen type I (Proteintech, 66761-1), rabbit anti–human TFRC (Zen BioScience, 619871), rabbit anti–human TGase2 (Abcam, ab64771), mouse anti–human TGase2 (Abcam, ab2386), rabbit anti–human RhoA (ABclonal, A13947), mouse anti–human RhoA (Santa Cruz Biotechnology Inc., sc-418), mouse anti–human IgA antibody (Southern Biotech, B3506B4), rabbit anti–human GAPDH (Bioworld Technology, AP0063), rabbit anti–human β-tubulin (ZEN Bio, 380628), rabbit anti–human Na+/K+ ATPase1 (ABclonal, A7878), or rabbit anti–Flotillin 2 (GeneTex, GTX114411) and detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies and ECL-Plus detecting system (GE Healthcare).
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