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Rneasy mini kit with oncolumn dnase digest

Manufactured by Qiagen
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The RNeasy Mini Kit with on-column DNase digest is a product designed for the purification of total RNA from various sample types. The kit utilizes a silica-membrane technology to efficiently capture and purify RNA, while an on-column DNase treatment step is included to remove any genomic DNA contamination.

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Lab products found in correlation

Rt2 first strand kit, by Qiagen (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Rt2 profiler pcr array, by Qiagen (2 mentions)

Spelling variants of this product

RNeasy Mini Kit (41418 citations) RNeasy kit (11240 citations) DNase (494 citations) RNeasy Mini (299 citations) Mini Kits (17 citations) DNase digest (14 citations) RNeasy Mini Kit with DNase (5 citations) DNase kits (3 citations) RNeasy kit Mini Kit (2 citations)

2 protocols using rneasy mini kit with oncolumn dnase digest

1

Heat Shock Protein Expression Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Human Heat Shock Proteins and Chaperones RT2 Profiler PCR Array (SABiosciences, Frederick, MD) was used to study the expression profile of 84 heat shock proteins according to the manufacturer’s procedure. Briefly, total RNA was extracted from cells transfected with HTT(Q74)GFP treated with DMSO or 25 uM of SEN177 inhibitor for 24h, using Trizol (Invitrogen) and further purified using RNeasy mini kit with oncolumn DNAse digest (Qiagen), cDNA was then synthesized using an RT2 First strand kit (SABiosciences) and real-time PCR was performed using 7900HT fast real time PCR system (Applied iosciences). Data were analysed with RT2 Profiler PCR Array data analysis software version 3.5.
Jimenez-Sanchez M., Lam W., Hannus M., Sönnichsen B., Imarisio S., Fleming A., Tarditi A., Menzies F., Dami T.E., Xu C., Gonzalez-Couto E., Lazzeroni G., Heitz F., Diamanti D., Massai L., Satagopam V.P., Marconi G., Caramelli C., Nencini A., Andreini M., Sardone G.L., Caradonna N.P., Porcari V., Scali C., Schneider R., Pollio G., O’Kane C.J., Caricasole A, & Rubinsztein D.C. (2015). siRNA screen identifies QPCT as a druggable target for Huntington’s disease. Nature chemical biology, 11(5), 347-354.
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2

Heat Shock Protein Expression Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Human Heat Shock Proteins and Chaperones RT2 Profiler PCR Array (SABiosciences, Frederick, MD) was used to study the expression profile of 84 heat shock proteins according to the manufacturer’s procedure. Briefly, total RNA was extracted from cells transfected with HTT(Q74)GFP treated with DMSO or 25 uM of SEN177 inhibitor for 24h, using Trizol (Invitrogen) and further purified using RNeasy mini kit with oncolumn DNAse digest (Qiagen), cDNA was then synthesized using an RT2 First strand kit (SABiosciences) and real-time PCR was performed using 7900HT fast real time PCR system (Applied iosciences). Data were analysed with RT2 Profiler PCR Array data analysis software version 3.5.
Jimenez-Sanchez M., Lam W., Hannus M., Sönnichsen B., Imarisio S., Fleming A., Tarditi A., Menzies F., Dami T.E., Xu C., Gonzalez-Couto E., Lazzeroni G., Heitz F., Diamanti D., Massai L., Satagopam V.P., Marconi G., Caramelli C., Nencini A., Andreini M., Sardone G.L., Caradonna N.P., Porcari V., Scali C., Schneider R., Pollio G., O’Kane C.J., Caricasole A, & Rubinsztein D.C. (2015). siRNA screen identifies QPCT as a druggable target for Huntington’s disease. Nature chemical biology, 11(5), 347-354.
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