Normal phase and reverse phase silica gel

For nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopic (HRESIMS) spectra, the Bruker Avance III and MicrOTOF-Q mass spectrometers have been selected. The following deuterated solvents were used to record the ¹H NMR (500, 600 MHz) and ¹³C NMR (125, 150 MHz) spectra: CDCl₃, DMSO-d₆, and CD₃OD. Normal-phase and reverse-phase silica gel (Merck) were used for thin-layer chromatography (TLC). The Himedia silica gel 60 was utilized for the column chromatography (CC) procedure. For the alpha-glucosidase inhibitory experiment, yeast Saccharomyces cerevisiae, α-glucosidase enzyme (Sigma-Aldrich, EC3.2.1.20) and the positive control acarbose (Sigma-Aldrich, EC260-030-7) were acquired. For the NO inhibition experiment, Griess' reagent (Sigma-Aldrich, EC215-981-2) and the positive control, L-NMMA (Merck, M7033), were utilized.

For nuclear magnetic resonance (NMR) and high-resolution electronspray ionization mass spectroscopic (HRESIMS) spectra, the Bruker Avance III and MicrOTOF-Q mass spectrometers were selected. The following deuterated solvents were used to record the NMR spectra (500 MHz for ¹H and 125 MHz for ¹³C): CDCl₃, DMSO‑d₆, and CD₃OD. Normal-phase and reverse-phase silica gel (Merck) were used for thin-layer chromatography (TLC). The Himedia silica gel 60 was utilized for the column chromatography (CC) procedure. Yeast Saccharomyces cerevisiae alpha-glucosidase enzyme (Sigma-Aldrich, EC3.2.1.20) and the positive control acarbose (Sigma-Aldrich, EC260-030-7), and Griess' reagent (Sigma-Aldrich, EC215-981-2) were utilized.