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Rat anti cd31 fitc

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Rat anti-CD31-FITC is a fluorescently-labeled antibody that binds to the CD31 (PECAM-1) antigen expressed on endothelial cells. CD31 is a cell adhesion molecule involved in the regulation of angiogenesis and vascular integrity. The FITC (fluorescein isothiocyanate) fluorescent label allows for the detection and visualization of CD31-positive cells.

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Lab products found in correlation

Rat anti cd45 fitc, by BioLegend (3 mentions) Rat anti ter119 fitc, by BioLegend (3 mentions) Y 27632 dihydrochloride, by Bio-Techne (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Rat anti cd49f pe, by BioLegend (2 mentions) Rat anti cd326 epcam apc cy7, by BioLegend (2 mentions) Goat anti trop2 apc, by R&D Systems (2 mentions) Rat anti esam fitc, by BioLegend (2 mentions) Rat anti cd44 fitc, by BioLegend (2 mentions) Rpm1 1640, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions) Fetal bovine serum (fbs), by Corning (2 mentions) Facscanto, by BD (2 mentions) Click it plus edu alexa fluor 647 flow cytometry assay kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using rat anti cd31 fitc

1

Cell Sorting and Antibody Staining

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Dissociated cells were stained with directly conjugated primary antibodies: rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend), goat anti-Trop2-APC (R &D Systems), rat anti-CD31-FITC (BioLegend), rat anti-CD45-FITC (BioLegend), and rat anti-Ter119-FITC (BioLegend) for 20 min on ice. Rat anti-ESAM-FITC (BioLegend) was also added to the Lin panel for some experiments. Rat anti-CD44-FITC (BioLegend) was used for analysis. Cells were stained in media containing RPM11640 (GIBCO), 10% FBS (Corning), 1x penicillin-streptomycin (GIBCO), and 10uM of the p160ROCK inhibitor Y-27632 dihydrochloride (Tocris Bioscience). Sorting was performed on a BD FACS Aria II (BD Biosciences) and flow cytometry analysis was performed on a BD FACS Canto (BD Biosciences).
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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2

Cell Sorting and Antibody Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissociated cells were stained with directly conjugated primary antibodies: rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend), goat anti-Trop2-APC (R &D Systems), rat anti-CD31-FITC (BioLegend), rat anti-CD45-FITC (BioLegend), and rat anti-Ter119-FITC (BioLegend) for 20 min on ice. Rat anti-ESAM-FITC (BioLegend) was also added to the Lin panel for some experiments. Rat anti-CD44-FITC (BioLegend) was used for analysis. Cells were stained in media containing RPM11640 (GIBCO), 10% FBS (Corning), 1x penicillin-streptomycin (GIBCO), and 10uM of the p160ROCK inhibitor Y-27632 dihydrochloride (Tocris Bioscience). Sorting was performed on a BD FACS Aria II (BD Biosciences) and flow cytometry analysis was performed on a BD FACS Canto (BD Biosciences).
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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3

Periosteal Cell Proliferation Assay

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Mice received 1.6 mg/kg EdU at 3 hr before sacrifice. Digested periosteal cells were stained with rat anti-Ter119 FITC (Biolegend, 116205), rat anti-CD31 FITC (Biolegend, 102509), rat anti-CD45 FITC (Biolegend, 147709), and rat anti-CD34 BV421 (BD Biosciences, 562608). EdU detection was carried out according to the manufacturer’s instructions (Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit, Thermo Fisher Scientific, C10424). Flow cytometry was performed by either LSR A or BD LSR Fortessa flow cytometer and analyzed by FlowJo v10.5.3 for MAC.
Yao L., Lu J., Zhong L., Wei Y., Gui T., Wang L., Ahn J., Boerckel J.D., Rux D., Mundy C., Qin L, & Pacifici M. (2023). Activin A marks a novel progenitor cell population during fracture healing and reveals a therapeutic strategy. eLife, 12, e89822.
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